Sequences from confirmation amplicon


Confirmation sequences for line 502D12 and hit At5g42720 are shown.
Line ID 502D12
Locus At5g42720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17132705
Primers used for PCR NU44 - 8474, show primer details
   
Sequence Name 16-K103931w-0022-502-D12C-NU44
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NU44: 5'-ACATACTTGTTCGCATTGTACGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K103931w-0022-502-D12C-NU44
TCTGACGAGCCTTCGGTTTGTTTAGACCTGATCTAACCATGACTTATGATATCGGGCTCA
CCAAGACTACCAATTATAACCAAGTAAGTTCTCGTTTCAAACATTAAATAGAAATATTGG
ATATGAACTTAAACGTATAGTTTTGATCAGATGTTCTATTTCATAAAAGTAAAAATTGTA
AACTGAAAACCCCGAACCGAATTTTGGTAACTAGTCAATGGATACTTAAAATTGAACATA
TATGATTCATATGATGTTAATTTAACTTGAGGCAAGTCCATGAAATGCACTACAATATAG
CCTCTTATTGATGTTAATTTAACTATCAATTATCTTTGTTACGTCTCCCAGACTTCCATG
GCTCCATTGTCACCAACAAGGCCACGTCTTCCACCAGCCGCGGCCCCGACGAGACAAACA
TTACCTTCTCCTCCGCAGATGATTCTTCCTTCACCAGTTACTCCCTCCGACAAAAACTCT
GGGCAAACCGATGTTCACAATTCCACTCCGCGCTCTGCCTCTCTGGCTCACATATGCCGC
TCTCTCTCACATTTATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATT
   
Sequence Name 32-K103932w-0022-502-D12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K103932w-0022-502-D12C-8409
CCGGACTGAGCCATATAAATGTGAGAGAGAGCGGCATATGTGAGCCAGAGAGGCAGAGCG
CGGAGTGGAATTGTGAACATCGGTTTGCCCAGAGTTTTTGTCGGAGGGAGTAACTGGTGA
AGGAAGAATCATCTGCGGAGGAGAAGGTAATGTTTGTCTCGTCGGGGCCGCGGCTGGTGG
AAGACGTGGCCTTGTTGGTGACAATGGAGCCATGGAAGTCTGGGAGACGTAACAAAGATA
ATTGATAGTTAAATTAACATCAATAAGAGGCTATATTGTAGTGCATTTCATGGACTTGCC
TCAAGTTAAATTAACATCATATGAATCATATATGTTCAATTTTAAGTATCCATTGACTAG
TTACCAAAATTCGGTTCGGGGTTTTCAGTTTACAATTTTTACTTTTATGAAATAGAACAT
CTGATCAAAACTATACGTTTAAGTTCATATCCAATATTTCTATTTAATGTTTGAAACGAG
AACTTACTTGGTTATAATTGGTAGTCTTGGTGAGCCCGATATCATAAGTCATGGTTAGAT
CAGGTCTAAACAAACCGAAGGCTCGTTCAGAACCTTTCCCGGGCTTGAGATTCTCGTCGT
ACAATGCGAACAAGTATG

Last Updated on 06.12.2018 16:11