Sequences from confirmation amplicon


Confirmation sequences for line 502G11 and hit At3g04000 are shown.
Line ID 502G11
Locus At3g04000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1036046
Primers used for PCR 14iv - 8474, show primer details
   
Sequence Name 77-K113077-0022-502-G11C-14iv
GenBank Accession LN513575 [GenBank]
Sequencing primer gene specific primer, 14iv: 5'-ATTACCACCAACTGTTCAAAGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K113077-0022-502-G11C-14iv
CTGATATCTCGAGCCGAGCCAGGTAAGTCGCTTTTCGACTGATGCAGAGCGAGTCTTCGA
GTCGCCGGTTCATATCTTGGTTAACTCAGCGGCTATAGCCGATCCTAATCATTCCACTAT
CTCAGACATGTCAGTAGAACTTTTTGATCGCATTATCAGGTTAATTTTAAGAAACTCATT
TATTATAAAGATTGTTTCATTTAGTTATTTTGCAACTCTATAACGTGAATTTAATTAGAT
AGTGATATTAATTAGATTCGTTGTTTGATATAACAGTGTGAACACAAGAGGTGCGTTTAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGGNTTTATT
   
Sequence Name 50-K113075-0022-502-G11C-8409
GenBank Accession LN507227 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K113075-0022-502-G11C-8409
TCCTAAACGCACCTCTTGTGTTCCACTGTTATATCAAACAACGAATCTAATTAATATCAC
TATCTAATTAAATTCACGTTATAGAGTTGCAAAATAACTAAATGAAACAATCTTTATAAT
AAATGAGTTTCTTAAAATTAACCTGATAATGCGATCAAAAAGTTCTACTGACATGTCTGA
GATAGTGGAATGATTAGGATCGGCTATAGCCGCTGAGTTAACCAAGATATGAACCGGCGA
CTCGAAGACTCGCTCTGCCTCATCGAAAAGCGACTTTACCTGGCTCGGCTCTGAGATATC
AGCCTTCACCACAATGACACGTGGACTTTTGCCGGCGACTTCCGCGTCCTTTGAACAGTG
GGTGGTAA

Last Updated on 06.12.2018 16:11