Sequences from confirmation amplicon


Confirmation sequences for line 503A05 and hit At3g52850 are shown.
Line ID 503A05
Locus At3g52850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19590605
Primers used for PCR IH08 - 8474, show primer details
   
Sequence Name 41-K023620-022-503-A05C-8409
GenBank Accession LN503930 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K023620-022-503-A05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGAAGCCATCATACTCATTCGT
TGGTTTTTTATGTAGATTTTATAATAGATTTATAATCTTTGTTAATGTGCATTTTCTGTA
TCCATATGTTATTTCACTTGGCAGCCTCTGGAGCTTTGCATTGTGGTATCAACAATGGAG
GATGCTGGAGAGAATCCCGAGGTGGCTTCACTTACTCTGCTTGCGTAGTAAGTATTTCGC
TTCTATGTCAATGTGTTTGAACAATCATCTTCTTAACCTAAAATAAAACTTCTTTCGTTC
TTGAAATGGATTTTTCAGGATGATCATTCAAAGGATTGCAAATGCCCACTTGGGTTCAAG
GGCGATGGAGTGAAGAACTGTGAAGGTACTTGCTAAGTATTATTTACAAAATATATGCAT
GTGGTCTACTTGCATATATAAAGAGCTCTATGTCGATTTATTTAGTAGGCATCACTAGTA
ACATGCTATTAGCCTATTGTTTATATGCATGCATGTCGGTCTAGAATATTTAGTGCAGAT
ACACAATTATCAGTGGAATTTTTTATTGTACTCAATCAAAGAAGTGAGATCATTGTTAAA
CGTCAAATTCAAATTCATAATCATATTCTTTTGATTGAATGTGTTAGATGTGGACGAGTG
CAAAGAAAAAACGGTGTGCCAGTGCCCAGAGTGTAAATGTAAAAACA
   
Sequence Name 42-K023620-022-503-A05C-IH08
GenBank Accession LN504318 [GenBank]
Sequencing primer gene specific primer, IH08: 5'-CAGATTCAAAAGCAGAAAGTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K023620-022-503-A05C-IH08
GACTCCAAATTAAGTATGGGGCTAGGAGGAAGGAAGATCTATTTCTTTTACCTATGCAAG
TGTCGTGCTCACGCATGTAAAGCAAACCGTTGCTGCAGCTGCATTCATAACTTCCCCAAG
TGTTTTTACATTTACACTCTGGGCACTGGCACACCGTTTTTTCTTTGCACTCGTCCACAT
CTAACACATTCAATCAAAAGAATATGATTATGAATTTGAATTTGACGTTTAACAATGATC
TCACTTCTTTGATTGAGTACAATAAAAAATTCCACTGATAATTGTGTATCTGCACTAAAT
ATTCTAGACCGACATGCATGCATATAAACAATAGGCTAATAGCATGTTACTAGTGATGCC
TACTAAATAAATCGACATAGAGCTCTTTATATATGCAAGTAGACCACATGCATATATTTT
GTAAATAATACTTAGCAAGTACCTTCACAGTTCTTCACTCCATCGCCCTTGAACCCAAGT
GGGCATTTGCAATCCTTTGAATGATCATCCTGAAAAATCCATTTCAAGAACGAAAGAAGT
TTTATTTTAGGTTAAGAAGATGATTGTTCAAACACATTGACATAGAAGCGAAATACTTAC
TACGCAAGCAGAGTAAGTGAAGCCACCTCGGGATTCTCTCCAGCATCCTCCATTGTTGAT
ACCACAATGCANAGCTCCAGAGGCTGCCAAGTGAAATAACATATGGA

Last Updated on 06.12.2018 16:11