Genotyping and primer details


Genotyping and primer details for 503A05 - At3g52850
Line ID 503A05
Locus At3g52850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19590605 of line 503A05 Chr3:19590605
   
Insertion allele amplicon 8474 - IH08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IH08: 5'-CAGATTCAAAAGCAGAAAGTCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19591370
   
wt amplicon (over InsPos) 03hn - IH08
wt amplicon start Chr3:19589973
wt amplicon end Chr3:19591370
wt amplicon size 1398 bp
Locus specific primer IH08: 5'-CAGATTCAAAAGCAGAAAGTCTGA-3'
2nd locus specific primer 03hn: 5'-TTTGCAGATTTGGAAACTAATGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11