Sequences from confirmation amplicon


Confirmation sequences for line 504D05 and hit At3g57310 are shown.
Line ID 504D05
Locus At3g57310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21208495
Primers used for PCR GA57 - 8474, show primer details
   
Sequence Name 11-K101423-0022-504-D05C-8409
GenBank Accession LN491048 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K101423-0022-504-D05C-8409
ATTTACATTGAATATATCCTGGCTCCACCAGAGCAACTGTATTATTCGTGGCATCTGTTA
TGATCGTCATGTCACCTCTGGCTCCAACCAGAGCAACTGTGGTTGGAGGTTGGGGTATAG
AGGAGAAAGCGGCATGCATCGTGACAAACCTCATGTCATGCCTGCCGGCGATACTCAAAG
GAAGTCAGCCACCGGCATATTGTTGCGAGATGCTGAAAGAGCAGCAGTCTTGTCTTTGTG
GTTACATAAAGTCTCCAACGTTTGGTCACTATGTCATCCCTCAAAATGCTCACAAACTTC
TAGCGGCTTGTGGTATTCTTTACCCTAAATGTTGAAACTCAAAACATTCAATCTTAGTGA
ATAGTGATTGATCATATATATCAAAATCTTATGTAATAATGATGATGATTGGGAATTTAT
TATCTCCATGTTCAGACTGATTCTATTATATATCCGTTTTTCTTATAAATTTGTTTTGGG
AAAATTTAGTGGATAAGTAATCAGGCTACCAATATTATAAGAATCGCTAGATTTTAATCA
ATAATTTTAGTAAATGATCACGCTNAGATGATTTTACCAAC
   
Sequence Name 51-K101426-0022-504-D05C-GA57
GenBank Accession LN507446 [GenBank]
Sequencing primer gene specific primer, GA57: 5'-TGTTGGTAAAATCATCTAAGCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101426-0022-504-D05C-GA57
TCTAGCGATTCTTATAATATTGGTAGCCTGATTACTTATCCACTAAATCTTTTCAAAACA
AATTTATAAGAAAAACGGATATATAATAGAATCAGTCTGAACATGGAGATAATAAATTCC
CAATCATCATCATTATTACATAAGATTTTGATATATATGATCAATCACTATTCACTAAGA
TTGAATGTTTTGAGTTTCAACATTTAGGGTAAAGAATACCACAAGCCGCTAGAAGTTTGT
GAGCATTTTGAGGGATGACATAGTGACCAAACGTTGGAGACTTTATGTAACCACAAAGAC
AAGACTGCTGCTCTTTCAGCATCTCGCAACAATATGCCGGTGGCTGACTTCCTTTGAGTA
TCGCCGGCAGGCATGACATGAGGTTTGTCACGATGCATGCCGCTNTCTCCTCTATACCCC
AACCTCCNACCACAGTTGCTCTGGTTGGAGCCAGAGGTGACATGACGATNATAACAGATG
CCACGAATAATACAGTTGCTCTGGTTGGAGCCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCCGGGAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGGAGAAAAGAAA
GAGTATTTACAATTTTTTTTCCATTTCAAAATGTAGATTTCCGCAC

Last Updated on 06.12.2018 16:11