Sequences from confirmation amplicon


Confirmation sequences for line 504E07 and hit At4g30180 are shown.
Line ID 504E07
Locus At4g30180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14769465
Primers used for PCR 45sm - 8474, show primer details
   
Sequence Name 44-K116491-0022-504-E07C-45sm
GenBank Accession LN505306 [GenBank]
Sequencing primer gene specific primer, 45sm: 5'-AGAGGCAAATCATAAACAGGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116491-0022-504-E07C-45sm
AACAGACCCTAGTGCAGTTTTCCGAGAAAAACACAAGCCACTTGGTTCCTGCACTCAAGA
AGCTCAACATGAACAAGAACTCTTCAAAACAAACCGTGAAGCATGAAGTAGATATGGCTT
TGGCTTTGTCTGCTCAAGAATTTGCATGGAGCCGTTTCTTGCTGCAGAAGCTATCGTCCT
CATCGAATCCAACCACTACCACTAGTTCTTCTTCCGATGGAATTCGGATTCTTGAAAGAC
CCGATAAAGAAGGCGGAAACGAAGAAGGAGGGATAGAGGAGAGACTGAGGGAATTGAAGA
AGCTTTTGCCAGGTGGGGAAGAGATGAATGTGGAAGAAATGTTGAGTGAGATTGGTAACT
ACATTAAATGTCTTGAGTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGTTTATTAT
   
Sequence Name 12-K116490-0022-504-E07C-8409
GenBank Accession LN491745 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116490-0022-504-E07C-8409
CATTTCAATTGAATAATCCTGCAACTCAAGACATTTAATGTAGTTACCAATCTCACTCAA
CATTTCTTCCACATTCATCTCTTCCCCACCTGGCAAAAGCTTCTTCAATTCCCTCAGTCT
CTCCTCTATCCCTCCTTCTTCGTTTCCGCCTTCTTTATCGGGTCTTTCAAGAATCCGAAT
TCCATCGGAAGAAGAACTAGTGGTAGTGGTTGGATTCGATGAGGACGATAGCTTCTGCAG
CAAGAAACGGCTCCATGCAAATTCTTGAGCAGACAAAGCCAAAGCCATATCTACTTCATG
CTTCACGGTTTGTTTTGAAGAGTTCTTGTTCATGTTGAGCTTCTTGAGTGCAGGAACCAA
GTGGCTTGTGTATTTTCTCGTGAAAACTGCACTAGGGTTCTTGTTTGGTTCAAGAGAAAA
CACTCGTTTCTTCCTGTTTAGAATTTGCCTCTA

Last Updated on 06.12.2018 16:11