Genotyping and primer details


Genotyping and primer details for 504E07 - At4g30180
Line ID 504E07
Locus At4g30180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14769465 of line 504E07 Chr4:14769465
   
Insertion allele amplicon 8474 - 45sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45sm: 5'-AGAGGCAAATCATAAACAGGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14769032
   
wt amplicon (over InsPos) 16tc - 45sm
wt amplicon start Chr4:14769032
wt amplicon end Chr4:14769785
wt amplicon size 754 bp
Locus specific primer 45sm: 5'-AGAGGCAAATCATAAACAGGAAGA-3'
2nd locus specific primer 16tc: 5'-ATTTTAGGCAGCACATCGTCTATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11