Sequences from confirmation amplicon


Confirmation sequences for line 504H09 and hit At5g11870 are shown.
Line ID 504H09
Locus At5g11870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3825878
Primers used for PCR 62ra - 8474, show primer details
   
Sequence Name 38-K115914-0022-504-H09C-62ra
GenBank Accession LN503107 [GenBank]
Sequencing primer gene specific primer, 62ra: 5'-CCTCGTCAACTACGACAACATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115914-0022-504-H09C-62ra
CAGGAAAAGAAAAAAGAGATTCCCGCCGCACGTTGTTCTAATCGAAAAAAGCGACTCTCA
ATAAATCTGTCTTCCCTTTTCTTTCTTAAACTGGAAGAAGATTAATCAATCAGACACAAC
AGAGATAACTGATTTATCCACTTCAAACAATACAAACCTTTAAAGTCTTCACCTTTATAG
AGAGAACTTTTAATGGTGTGGTTGACGAATCAACAGATAGGGAGATGGAAAAGGAAACGA
ATTTTGGTTGTGGGATCGTTCCTCTGTTGGTCAATCATCATGTTCATCACTCCCAAAGTT
CCTCTCGATTCATTTCGCCATCATATCTTCGCCGATAAACGCAATTTCATGGAACGAGAG
ACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTC
   
Sequence Name 12-K115913-0022-504-H09C-8409
GenBank Accession LN491728 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115913-0022-504-H09C-8409
CTCTCGTTCATGAAATTGCGTTTATCGGCGAAGATATGATGGCGAAATGAATCGAGAGGA
ACTTTGGGAGTGATGAACATGATGATTGACCAACAGAGGAACGATCCCACAACCAAAATT
CGTTTCCTTTTCCATCTCCCTATCTGTTGATTCGTCAACCACACCATTAAAAGTTCTCTC
TATAAAGGTGAAGACTTTAAAGGTTTGTATTGTTTGAAGTGGATAAATCAGTTATCTCTG
TTGTGTCTGATTGATTAATCTTCTTCCAGTTTAAGAAAGAAAAGGGAAGACAGATTTATT
GAGAGTCGCTTTTTTCGATTAGAACAACGTGCGGCGGAATCTCTTTTTTTCTTTTTCCTT
GAGTCTGACGGGTACGACGACGACGATCGATCTCTAATGTTGTCT

Last Updated on 06.12.2018 16:11