Sequences from confirmation amplicon


Confirmation sequences for line 506B12 and hit At5g33280 are shown.
Line ID 506B12
Locus At5g33280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:12550449
Primers used for PCR BB82 - 8474, show primer details
   
Sequence Name 12-K104123-0022-506-B12C-BB82
GenBank Accession LN491551 [GenBank]
Sequencing primer gene specific primer, BB82: 5'-CCATCATCATAGGTAGTAGCAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104123-0022-506-B12C-BB82
AGTGGAAACTGTCATCCTCATTGTTCCGCCAAGAAAGGATGCAGCACCGAGCACAGCAAA
CAGACCATGATTAAGATTTGAGTTTGAACCAAGCAGCATCCCTACAAACCGTCCATACGA
TGCTCCTGTTACAATAACTGGCACAAAGAGCCCTGCTGGAGCAACAATGCCATAGCTAAA
GATACTGAGGAAGAAGCAGGTGACAAAAAATACGAGAACCGAAAAATAATGAAACTCGAA
GTCAGTGTTTTTGCTGAAAAGGTTTTTGATGGCATCATCATTTGTGTTGAAAATAAGGCT
AGCTAGATCATTGTAGTGACCAGGAGGGCATTGGTATTTCTTAAAGTTTCCAGACCGTCC
AATTGTAGGGCACTCCTCTAAGGCATCGACAGGACAAGGTTGGCATGATGCAAGAAATGG
TAATCCGAAAAGAAGGCAGGACGTGAAAATCGATATCGCACAAGCGAGGAGGATTTTCCA
AGTAACCCCTTTCCTGGACAGAAAAAACTGTTAGAAATCAAGAAAACATTTACTTGTGTG
TATGTGTCTGTAGTTGTAAGCTTACTCATATATGTAGTTGTAAGCTCGCAGAACCTTATC
CAGTAAGAAGTTGTATAAGCTTCCTAAAATACCACCAACAACCCCNAGAAGAAGAACAGG
AAGTACATCCCCTAAATGATATGACGCANTTTCTGAGTAGACATCANACATTATTAAGCC
TCCTTTACCANATAACCCACACTTTCCACTTAAACACACATCGATAAGAGCTCTGAGAAC
TATNGCCACAACAGCTGTCGAAAAGAAGATTCTCCATAANAGTGCACTCC
   
Sequence Name 28-K104124-0022-506-B12C-8409
GenBank Accession LN498817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104124-0022-506-B12C-8409
GCCATTTACAATTGAATATAGCCATTTACTTTTAGAATGGGGTTCCCGGACATGTAGATT
GCTGATCCGTTGTAGGAGTGCACTTTTATGGAGAATCTTCTTTTCGACAGCTGTTGTGGC
AATAGTTCTCAGAGCTCTAATCGATGTGTGTTTAAGTGGAAAGTGTGGGTTATTTGGTAA
AGGAGGCTTAATAATGTTTGATGTCTACTCAGAAAATGCGTCATATCATTTAGGGGATGT
ACTTCCTGTTCTTCTTCTTGGGGTTGTTGGTGGTATTTTAGGAAGCTTATACAACTTCTT
ACTGGATAAGGTTCTGCGAGCTTACAACTACATATATGAGTAAGCTTACAACTACAGACA
CATACACACAAGTAAATGTTTTCTTGATTTCTAACAGTTTTTTCTGTCCAGGAAAGGGGT
TACTTGGAAAATCCTCCTCGCTTGTGCGATATCGATTTTCACGTCCTGCCTTCTTTTCGG
ATTACCATTTCTTGCATCATGCCAACCTTGTCCTGTCGATGCCTTAGAGGAGTGCCCTAC
AATTGGACGGTCTGGAAACTTTAAGAAATACCAATGCCCTCCTGGTCACTACAATGATCT
AGCTAGCCTTATTTTCAACACAAATGATGATGCCATCAAAAACCTTTTCAGCAAAAACAC
TGACTTCGAGTTTCATTATTTTTCGGTTCTCGTATTTTTTGTCACCTGCTTCTTCCTCAG
TATCTTTAGCTATGGCATTGTTGCTCCAGCAGGGCTCTNTGTGCCAGTTATTGTAACANG
AGCATCGTAAGGACGGTTTGTAGGGATGCTGCTTGGTTCANACTCAAATCTTAATCATGG
TCTGTTTGCTGTGCTCGGTGCTGCATCCTTTCTTGGCGGAACAATGAGGATGACAGTCTC
CACTTGTGTTATTCTCCTTGAGCTGACCAACA

Last Updated on 06.12.2018 16:11