Sequences from confirmation amplicon


Confirmation sequences for line 506E01 and hit At5g50040 are shown.
Line ID 506E01
Locus At5g50040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20357789
Primers used for PCR NG61 - 8474, show primer details
   
Sequence Name 44-K102366-0022-506-E01C-8409
GenBank Accession LN505135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K102366-0022-506-E01C-8409
CATTTCTTATGCCGATAAGTCTGCGGGTTTCACCGGAAATGCGGCAAAGGAGAATCCAAC
CTTGAAGACGCAATTTGCAGCGAGTCAAGATGCCTTTGTGACCATTAGCAAGTCTCTCAA
GAGTGCTGCTTCGGAACTCAAGATATCGCCAGACACTGCCAACTATGATGTCATGGTTTG
TAGTGACAGCATAGCGACGGTGAAGAATTTGGTAGAGAAAAATACAGACAATTCGTCAAA
GACTGTTATGACGATGACATTGATGATGGAGAAACTTCTTACAATCGCCGTTGGAGCCAC
TGTCGCTGTTGGCGGATAAAGACACGATAATTAAA
   
Sequence Name 44-K102367-0022-506-E01C-NG61
GenBank Accession LN505136 [GenBank]
Sequencing primer gene specific primer, NG61: 5'-TAATTATCGTTGTCTTTATCCGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K102367-0022-506-E01C-NG61
AGAAGTTTCTCCATCATCAATGTCATCGTCATAACAGTCTTTGACGAATTGTCTGTATTT
TTCTCTACCAAATTCTTCACCGTCGCTATGCTGTCACTACAAACCATGACATCATAGTTG
GCAGTGTCTGGCGATATCTTGAGTTCCGAAGCAGCACTCTTGAGAGACTTGCTAATGGTC
ACAAAGGCATCTTGACTCGCTGCAAATTGCGTCTTCAAGGTTGGATTCTCCTTTGCCGCA
TTTCCGGTGAAACCCGCAGACTTATCGGCATAAGAAATGGCTAGGTTGAGCGTGGCCGTA
GCTGCTTGAAACTGCAATGAGTATGATGGTCAAT

Last Updated on 06.12.2018 16:11