Sequences from confirmation amplicon


Confirmation sequences for line 508G12 and hit At1g26945 are shown.
Line ID 508G12
Locus At1g26945 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9352264
Primers used for PCR BM72 - 8474, show primer details
   
Sequence Name 16-K104415-0022-508-G12C-BM72
GenBank Accession LN493419 [GenBank]
Sequencing primer gene specific primer, BM72: 5'-ACCGATAATCTCCTCAGCTTGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K104415-0022-508-G12C-BM72
ATAGTAAAAAAGGAAACGGACCGTGTCATTAATCATAAATATTAGAGGATCTCATGATAC
ACAGGCTACTATACGCCAGGTCTGGACCCTCAGATACAATTAACATGCATACATACATAC
ATAACCATATATTAAATATAAATATATGAGTTGGCCTAACAATGACCCGAAACCCTAGCT
ATATCTCATAATATATGATGTAGAAGTAAACAGACGATACCCATAATAAGATAAATTTGG
TGGCCGGAAACGGATGATTAATAGCTCTCAGATTAAGTAATGCGGATTTAATAATTAAGC
AAGCTCCTAATGATGGCTGCTTCGGCGCTGTTGTCGTCCGTCGAAGCCAAGAGTTCCGAC
AAACGGTCACTGAGGTCATCAACCTCTCTGTGTAAGTTCCTGATGTAGTTGCAAGTCTCT
TGTAGTACCTTAGATGCTGACACCTGTCCAATAGTTTAAACATTTTACAATTAGTTTTAA
AGTACAAAAAACGATCATGTAACAATTAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 32-K104416-0022-508-G12C-8409
GenBank Accession LN500571 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K104416-0022-508-G12C-8409
ATTTACAATTGAATATATCCTAATTGTTACATGATCGTTTTTTTGTACTTTAAAACTAAT
TGTAAAATGTTTAAACTATTGGACAGGTGTCAGCATCTAAGGTACTACAAGAGACTTGCA
ACTACATCAGGAACTTACACAGAGAGGTTGATGACCTCAGTGACCGTTTGTCGGAACTCT
TGGCTTCGACGGACGACAACAGCGCCGAAGCAGCCATCATTAGGAGCTTGCTTAATTATT
AAATCCGCATTACTTAATCTGAGAGCTATTAATCATCCGTTTCCGGCCACCAAATTTATC
TTATTATGGGTATCGTCTGTTTACTTCTACATCATATATTATGAGATATAGCTAGGGTTT
CGGGTCATTGTTAGGCCAACTCATATATTTATATTTAATATATGGTTATGTATGTATGTA
TGCATGTTAATTGTATCTGAGGGTCCAGACCTGGCGTATAGTAGCCTGTGTATCAGGAGA
TCCTCTAATATTTATGATTAATGACACGGTCCGTTTCCTTTTTTACTATACTGATCTACT
GTTATAAGTTTCGTTTATCATATCTTTTTCTATAATTAACAAGCTGAGGAGATTATCG

Last Updated on 06.12.2018 16:11