Sequences from confirmation amplicon


Confirmation sequences for line 509E06 and hit At5g46370 are shown.
Line ID 509E06
Locus At5g46370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18811622
Primers used for PCR MJ70 - 8474, show primer details
   
Sequence Name 16-K029635-022-509-E06C-8409
GenBank Accession LN493310 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K029635-022-509-E06C-8409
GCCATTTACAATTGAATAATCCTGACCGGAGAACAATGACGTCACAACTCCGATGCCAAT
AACTCCATTGGAACTTAAAAACCGATTAATCTTCGGTTCATTCGTTCGTTCTAGAAAAGA
ATCATCTCTTCCCATCGATGCTCTCTCTCAAAACCCTTCTACGTCATCCTCCGCCACAAC
CTCATTCTCCGATTCCACAGACCTCCTTCTCCCACTTACGGAGCCTAATAAACCGGTTCG
GAAATCTAAACCGACCATCAATTTCCACCGGTCTAAAACAGCTCCTGCTATGGCTGCAAT
CAACAATATATCTCATCCCAATGATCCCAAAACAGATCAGCAATCTGATTCGAAAACTAT
TGTAAACCAAGCTGTTGCTTTACTTGTTGTGTATCTATCCTTAGGTGTGCTTATCTATTG
GTTGAATCGAGATAGCTACAATGTGAAGCAGACTCATCCTGTTGTTGATGCTTTGTACTT
TTGTATTGTTACGATGTGTACGATTGGGTATGGAGATATAACGCCGGATAGCGTGGTGAC
CAAGCTGTTTTCGATATT
   
Sequence Name 17-K029635-022-509-E06C-MJ70
GenBank Accession LN493726 [GenBank]
Sequencing primer gene specific primer, MJ70: 5'-AAAAATATCGAAAACAGCTTGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K029635-022-509-E06C-MJ70
GTCTGCTTCACATTGTAGCTATATCGATTCAACCACTAGATAAGCACACCTAAGGATAGA
TACACAACAGGTAAAGCAACGGCTTGCTTTACAATGAGGTTTCGAACCAGATTGTTGATC
TGTTTTGGGATCATTGGGATGAGATATATTGTTGATTGCAGCCATAGCAGGAGCTTGTTT
TAGACCGGTGGAAATTGATGGTCGGTTTAGATTTCCGAACCGGTTTATTTGGCTCCGTAA
GTGGGAAAAGGAGGTCTCTGGAATCAGAGAATGTAGGTTGTGGAAGACTATTACGTATAA
TGGTTTTGAGATACAGCATCGATGGGAAGAGACGATTCTTTTCTAGAACGAACAAATGAA
CCTAAGATTAATCGGTTTTTTAGTTCCAATGGATTTTTT

Last Updated on 06.12.2018 16:11