SimpleSearch - Line and FST details


Line specific information

 
Line ID 509E06
Vector Used pAC161
Line Availability available as T3 set from NASC (N448822)
Segregation Analysis 50:49:38
Confirmed for Hit At5g46370
Parent of DUPLO pair none
Parent of pair(s) 3154, 8113

Gene hit F14I23

 
Sequence (A. th genome BLAST matches underlined) 
>45-K019531-022-509-E06-8409
TAATGATGATTAGTGTATTATTTGATGCCGATGCTATAGCTCACTATGTATATTAACTCT
GATTATTTCGTGGTTACTATTTGTATACGGGATATGGGATACTTCCGAGTTAGTATGACT
CAATTACTCAGAGNCGTATTACTCANNNTTCTTTTNATGAAAAAAGTTAATATTANGCCT
TATTTTTTTCTATATAATTTGATAATAACTTTTTTTAGCTTCATGACATTAATGTGATTT
TCAAACTCTAAAGATATTATACCAAAAATGCATAAATAAGCTTATTTAAGACCGTTAGGT
ATTTGGACTAATAAGTAGGATAATTATTTTTTTTCTTATCAGATGCGCACATGTCAGCTA
AACAAACCCTTATAAAAAAATGTGGG
GenBank Accession BX534450 [GenBank]
Graphic View Graphic view of sequence BX534450 of line 509E06
Predicted Position of Insertion Chr5:9911312 - go to primer design
BLAST e Value 3e-48
Hit Clone Code (BAC ID) F14I23
Insertion Classification Genome Hit
Confirmation Status unknown

Gene hit At5g46370

 
Sequence (A. th genome BLAST matches underlined) 
>45-K019519-022-509-E06-8409
ATGAGCATTTACATTGATATATCCTGACCGGAGACAATGACGTCAGCAATTCCGATGCCA
ATAACTCCATTGGAACTTAAAAACCGATTAATCTTCTTGTTCATTCGTACGTGCCTATGG
GATCCTCCCTATAGTGAGACTCCCTATACCGAGCTATAGTGATCTACACTGAATTGGTAT
ATCCCGTTTGGACGTGAATGTATACACCCCGAATTAACCCCTTCCGAAATACAATTAATT
GTCTAGTGCTTTCTGCGATATTGACGACCGATCGAAATTTGCCGAATCATCATAATGAAC
CTTCATTTAATACTAACACTGCGNA
GenBank Accession BX534449 [GenBank]
Graphic View Graphic view of gene At5g46370
Predicted Position of Insertion Chr5:18811614 - go to primer design
BLAST e Value 1e-22
Hit Clone Code (BAC ID) MPL12
Hit Gene Code At5g46370 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Ca2 activated outward rectifying K channel 2
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit FR811373 [GenBank]


Last Updated on 10.06.2021 13:37