Sequences from confirmation amplicon


Confirmation sequences for line 510A05 and hit At1g34050 are shown.
Line ID 510A05
Locus At1g34050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12394380
Primers used for PCR CC79 - 8474, show primer details
   
Sequence Name 21-K105918-0022-510-A05C-CC79
GenBank Accession LN495661 [GenBank]
Sequencing primer gene specific primer, CC79: 5'-ATTGTGGCTCAGATATTAGCGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K105918-0022-510-A05C-CC79
CTGCCTTCGTTGTTGCTTGCCGCTACACTAACCCCGATGTCGCTAGTCTTATATTGGAAG
AAACAAGTTCTATCACAATCGGGGAATTTTATGCAACGTTTGTTCTTGGAGAATATACGG
GTTAGTGTGTTTATTTATTTACTTATAAAACTATACATAATAAGCAACCAAGGTCATGCA
ACTTGACAACTTGTAGTGTTACAAAAGAATGTTTTCTATCAAGTTAGTATTAGAGCTAAT
TATGACTTGATAGTATACGGAACGCGTAACTCAAATCTTGTCTGGTGGATAAGCTTTGAT
TGTAAGTCAAATTTAGTTCACTAGATTTTTCACAAAGACAAAGCAAATTTTCACTAATAC
AAAATTTAAATTCGTACATGAAAAAGAGAAAAATAATTGAACTTTTATTAATTCTCTTGA
ACAAAGTGTAAATAGTTAAGTGTTAAAGACTTTCATGCAAAATCTAGAGGTCGCGTATTT
GAACACTTGGGAGGGGACTAATAAAAAATTCTGGTCTCTACATGATACCTTACTGATCGT
TATTACTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTC
   
Sequence Name 21-K105919-0022-510-A05C-8409
GenBank Accession LN495662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K105919-0022-510-A05C-8409
GCCATTTACAATTGAATATATCCTGATGAGTAATAACGATCAGTAAGGTATCATGTAGAG
ACCAGAATTTTTTATTAGTCCCCTCCCAAGTGTTCAAATACGCGACCTCTAGATTTTGCA
TGAAAGTCTTTAACACTTAACTATTTACACTTTGTTCAAGAGAATTAATAAAAGTTCAAT
TATTTTTCTCTTTTTCATGTACGAATTTAAATTTTGTATTAGTGAAAATTTGCTTTGTCT
TTGTGAAAAATCTAGTGAACTAAATTTGACTTACAATCAAAGCTTATCCACCAGACAAGA
TTTGAGTTACGCGTTCCGTATACTATCAAGTCATAATTAGCTCTAATACTAACTTGATAG
AAAACATTCTTTTGTAACACTACAAGTTGTCAAGTTGCATGACCTTGGTTGCTTATTATG
TATAGTTTTATAAGTAAATAAATAAACACACTAACCCGTATATTCTCCAAGAACAAACGT
TGCATAAAATTCCCCGATTGTGATAGAACTTGTTTCTTCCAATATAAGACTAGCGACATC
GGGGTTAGTGTAGCGGCAAGCAACAACGAAGGCAGTTTCTCCTCGTCCGTTGACCGGGGT
GAACTCAGCATATCCNGACGC
Primers used for PCR CC78 - 8474, show primer details
   
Sequence Name 22-K105918-0022-510-A05C-CC78
GenBank Accession LN496114 [GenBank]
Sequencing primer gene specific primer, CC78: 5'-CTAGATGGAAGACGGTTTCTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K105918-0022-510-A05C-CC78
CTCCTTCAGAATTGGAATTGAGCATTTCATGGCCGCCAAATGCAAAGGTGTGAAACCATC
TTTGTTGACCCTCTCTGCAAGGCTTTCATCAATCTCTAGCAACATTTTTGTTATCTCGAG
GTTGTTGGCGTTGCACGCATGATGCAATGGGGTTGACAATTCTCCATCGGCATTCCAAGC
TAGTTTTGGGAACCTCTCTAGCATTCTCCTTGCGATATCTGATCATATGAACACATGTCA
GTACAGTGATCGACATGTTTCCATGAACACATGCTGAGTTCATATTTATTAGTTCTTTTT
TCTTTTTTTGAATTACTAAGAGGTTTAGACCTACGCCCGTACATCTCCTCAGGCCAGAGC
CTGACGAAAGCCTCTTACTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 22-K105919-0022-510-A05C-OriL
GenBank Accession LN496115 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K105919-0022-510-A05C-OriL
ATTCGGACATGAGCCATTTACAATTGAATATATCCTGATGAGTAAGAGGCTTTCGTCAGG
CTCTGGCCTGAGGAGATGTACGGGCGTAGGTCTAAACCTCTTAGTAATTCAAAAAAAGAA
AAAAGAACTAATAAATATGAACTCAGCATGTGTTCATGGAAACATGTCGATCACTGTACT
GACATGTGTTCATATGATCAGATATCGCAAGGAGAATGCTAGAGAGGTTCCCAAAACTAG
CTTGGAATGCCGATGGAGAATTGTCAACCCCATTGCATCATGCGTGCAACGCCAACAACC
TCGAGATAACAAAAATGTTGCTAGAGATTGATGAAAGCCTTGCAGAGAGGGTCAACAAAG
ATGGTTTCACACCTTTGCATTTGGCGGCCATGAAATGCTCAATTCCAATTCTGAAGGAGT
TTTCGGATAAGGCTCCAAGATATTTCGACATACTAACACCAGCAAAAGAAACCGTCTTCC
CATCTA

Last Updated on 06.12.2018 16:11