Sequences from confirmation amplicon


Confirmation sequences for line 510E04 and hit At1g52580 are shown.
Line ID 510E04
Locus At1g52580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19588661
Primers used for PCR 67zg - 8474, show primer details
   
Sequence Name 32-K125801-0022-510-E04C-67zg
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 67zg: 5'-AGATATCGAAAACGGACCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K125801-0022-510-E04C-67zg
GCGCCATTCCGAGACATGGGTGCGTGGCTTATGCCTCTCATTTTAGCAGCCAACTTCGTC
ACATTCGCTACCACCATGTATGTGAACGATTGCCCTGCAAGATCAGATGAGTGTCTCTTA
TTTGATGTCCTTGGAAGGCTTTCGTTTCAACCGATTAAGGAGAATATGCTACTCGGTCCT
TCTATTCCCACGTAAGGAAGGATTTCTTGATTCCATAATGGTTTTTTTCTCTCCGGTTAG
AACAAGGAATCTATTAAAAACTGGTTCAATACTTACAGAATGCATCTTTGTTGTTGATTC
AGGCTGAGAAAACTCGGAGCTCTCGAAAGGAGACTTGTGGAGGAAGGTGAGAGATGGCGT
CTCATCTCTTGCATATGGCTTCATGGTGGTTTCCTTCACTTGATGGCTAACATGATCAGC
CTCATGTGTATCGGAATGCGCCTTGAACAAGAATTTGGATTCAGTAAGACCCTTTAAACC
CCTCTATTTTCCGTCTCCTGAATTTTCTGTGATCTGATTTAAGAGTTTGGTGTTACAGTG
AGAATCGGAGCTTTGTATGTGATCTCCGGGCTTGGAGGAAGCCTGGTGTCTTGCTTAACA
GATTCCCAAGGAGAACGGGTATCAGTCGGAGCATCTGGTGCTTTGTTCGGATTACTCGGT
GCAATGCTCTCAGAGCTCATCACAAACTGGACAATCTATGANAATAAGGTAAAAAGATTA
TTCATTTTCAATATGGTGCATTATTGAAAGCTGATCTCAAACTTTTTTGTAGTGCACCGC
ATTTATGACATTAATCCTGATCATTGTACTGAATCTAAGCGTCGGATTCTTACCACGTGT
GGACAATTCTGCTCATTTTGGAGGATTCCTAGCTGGTTTCTTCCTCGGGTTCGTCCTTCT
TCTCC
   
Sequence Name 46-K125800w-0022-510-E04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K125800w-0022-510-E04C-8409
TTTCATTGAACCAGGAGGAATGTATTTTGGGTTCACGTATCCGTACTGCGGACGGAGAAG
AAGGACGAACCCGAGGAAGAAACCAGCTAGGAATCCTCCAAAATGAGCAGAATTGTCCAC
ACGTGGTAAGAATCCGACGCTTAGATTCAGTACAATGATCAGGATTAATGTCATAAGTGC
GGTGCACTACAAAAAAGTTTGAGATCAGCTTTCAATAATGCACCATATTGAAAATGAATA
ATCTTTTTACCTTATTTTCATAGATTGTCCAGTTTGTGATGAGCTCTGAGAGCATTGCAC
CGAGTAATCCGAACAAAGCACCAGATGCTCCGACTGATACCCGTTCTCCTTGGGAATCTG
TTAAGCAAGACACCAGGCTTCCTCCAAGCCCGGAGATCACATACAAAGCTCCGATTCTCA
CTGTAACACCAAACTCTTAAATCAGATCACAGAAAATTCAGGAGACGGAAAATAGAGGGG
TTTAAAGGGTCTTACTGAATCCAAATTCTTGTTCAAGGCGCATTCCGATACACATGAGGC
TGATCATGTTAGCCATCAAGTGAAGGAAACCACCATGAAGCCATATGCAAGAGATGAGAC
GCCATCTCTCACCTTCCTCCACAAGTCTCCTTTCGAGAGCTCCGAGTTTTCTCAGCCTGA
ATCAACAACAAAGATGCATTCTGTAAGTATTGAACCAGTTTTTAATAGATTCCTTGTTCT
AACCGGAGAGAAAAAAACCATTATGGAATCAAGAAATCCTTCCTTACGTGGGAATAGAAG
GACCGAGTAGCATATTCTCCTTAATCGGTTGAAACGAAAGCCTTCAAGGACATCAAATAA
GAGACACTCATCTGATCTTGCAGGCCAATCGTTCACATACATGGTGGTAGCGAATGTGAC
GAAATTGGCTGCTAAATG

Last Updated on 06.12.2018 16:11