Sequences from confirmation amplicon


Confirmation sequences for line 511F01 and hit At5g42860 are shown.
Line ID 511F01
Locus At5g42860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17183846
Primers used for PCR KN59 - 8474, show primer details
   
Sequence Name 02-K103735-0022-511-F01C-KN59
GenBank Accession LN487000 [GenBank]
Sequencing primer gene specific primer, KN59: 5'-AGATGCTGGAGGTATAGGAACTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103735-0022-511-F01C-KN59
GTTGTACCGTAACACTGGTACTTTCTTTGGTGTTCATGTCACTTCTTCTCCGATTGATCT
TAGCTTCTCGCAGATCACTATTGGCTCTGGCTCTGTAAGTTCTAAAATCTGAAACTTCTT
TAAAATGTGTCTAATTCATCTTCATCAATGTTGTTGTCATGTTGCTATCACCAGATTCTC
TTTGTTTCTTTTTTTTTTTATGAAAAATGTATAACCAAACCTTTTTCGGTTTATTTTGAA
AAAAATTTCGGTTCGGTTTATTTTTTGGTTAGTCTTTGATCAATGTTGTCGGTGGCTATT
ATCACATGGGGTTGATCAGGAGTGCACATTTTGGTGTTTATTTTTAAAATAAAAACACCA
ATGATAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGATTATGATGGTCAA
TATGGAAAAAAAAAAAAAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAAATGTCCC
CACCGTTATAAAA
   
Sequence Name 18-K103736-0022-511-F01C-8409
GenBank Accession LN494273 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K103736-0022-511-F01C-8409
GCCATTATCATTGCTGTTTCTATTTTAAAACTAAACACCAAAATGTGCACTCCTGATCAA
CCCCATGTGATAATAGCCACCGACAACATTGATCAAAGACTAACCAAAAACTAAACCGAA
CCGAACTTTTTTCCAAACTAAACCGAAAAAGGTTCGGTTATACATTTTTCATAAAAAAAA
AAAAAAACAAAAAAAATCTGGTGATAGCAACATGACAACAACATTGATGAAAATGAATTA
AACACATTTTAAAAAATTTTCAAATTTTAAAACTTACAAAGCCAAACCCAATAGTGATCT
GCAAAAAGCTAAAATCAATCGGAAAAAAAGTGACATGAACCCCAAAAAAAGTACCAGTGT
TACGGTACAACATTCTCAGGGTCGCGTTCATAGGGATCATATCATTTCCAATAC
Primers used for PCR AL04 - 8474, show primer details
   
Sequence Name 26-K118808-0022-511-F01C-8409
GenBank Accession LN498146 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118808-0022-511-F01C-8409
TGGATATATATTTTTAAAAGTTTGATCTAGTGTGGTTGTTACTAAATTCAATTTTGCTTT
ATTTGACATAGACATTTTCTTAACATTTGGTTTTACAATATAGTTTTGATGACCCACTTC
CAATTTAATTTGATCGTTATTCTTTTTACTTTTGTAGATTAAGAAGTTTTATCAGTCAAG
GAAAAGCCAAAGAACAGTGGTGGTGAATGTACTTGGAGACAAGATTCCACTCTATGGAAG
TGGCTCAACATTAGTCCCACCACCACCTCCTGCACCAATTCCAAAACCCAAGAAGAAGAA
AGGACCTATCGTTATCGTCGAACCACCAGCGCCTCCTGCTCCAGTACCGATGAGGCTAAA
CTTCACCGTTAGATCTCGAGCTTACGTGTTGGGGAAGTTAGTTCAACCTAAGTTTTACAA
GAGAATCGTGTGCTTGATTAATTTCNAACACAAG

Last Updated on 06.12.2018 16:11