Sequences from confirmation amplicon


Confirmation sequences for line 511G10 and hit At4g17180 are shown.
Line ID 511G10
Locus At4g17180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9647066
Primers used for PCR 25wx - 8474, show primer details
   
Sequence Name 39-K118924-0022-511-G10C-25wx
GenBank Accession LN503529 [GenBank]
Sequencing primer gene specific primer, 25wx: 5'-TGCCTAGTCCTAAGTTGAGACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118924-0022-511-G10C-25wx
CTCTCAAGTTACAGGCAGTGTGCTTTTCGCTCCTTCGTCAAGCAAACCGAAAAGATATAC
ATCCGCAGGAGGAGATCCAGGCCTGAGCGGTGTTCCTTTGTTGCTCAACACATGGCTAAT
GAGCCCTTGATTAAAAACCCGGGCTGCTGTGAGATTAGCACCAACTGCTCCATCTGTAGG
CCAACCAATCTCACCAATAACTATAGGCATTTGACCATATCCAAGCTTGGTGAGAGCAGC
AACAAGAGTGTCAAAGTTCCCATCAAATGCATTATAGTAAGTGTTAGGCCCATCCGGAAC
CGGATGACTACTCCCCTCAAAAAAGGCATAGTCTTGTGGAAAATCCGAGTTCCCATAGAG
ACTAAGGAAAGGATAGATATTAACCACAAAAGGAGAGCCATTGGAGTTGAGAAACGAAAC
CAATTGAGTCATAATCTGTGTAAGCTCAGGCCGGAACATCCCCTGAGAGGGAACATTAGA
CTGATACGCATCTGCGTTGCAAGGAACCACCAACTTCACAAACCGTCAATATGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATTAT
   
Sequence Name 15-K118923-0022-511-G10C-8409
GenBank Accession LN493201 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118923-0022-511-G10C-8409
GAGTTGGTGGTTCCTTGCAACGCAGATGCGTATCAGTCTAATGTTCCCTCTCAGGGGATG
TTCCGGCCTGAGCTTACACAGATTATGACTCAATTGGTTTCGTTTCTCAACTCCAATGGC
TCTCCTTTTGTGGTTAATATCTATCCTTTCCTTAGTCTCTATGGGAACTCGGATTTTCCA
CAAGACTATGCCTTTTTTGAGGGGAGTAGTCATCCGGTTCCGGATGGGCCTAACACTTAC
TATAATGCATTTGATGGGAACTTTGACACTCTTGTTGCTGCTCTCACCAAGCTTGGATAT
GGTCAAATGCCTATAGTTATTGGTGAGATTGGTTGGCCTACAGATGGAGCAGTTGGTGCT
AATCTCACAGCAGCCCGGGTTTTTAATCAAGGGCTCATTAGCCATGTGTTGAGCAACAAA
GGAACACCGCTCAGGCCTGGATCTCCTCCTGCGGATGTATATCTTTTCGGTTTGCTTGAC
GAAGGAGCGAAAAGCACACTGCCTGGTAACTTTGAGAGGCATTGGGGAATCTTCTCTTTC
GATGGACAGGCCAAGTATCGTCTCAACTTAGAACTAGGC

Last Updated on 06.12.2018 16:11