Sequences from confirmation amplicon


Confirmation sequences for line 511G12 and hit At1g23880 are shown.
Line ID 511G12
Locus At1g23880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8436898
Primers used for PCR 31cp - 8474, show primer details
   
Sequence Name 69-K108745-0022-511-G12C-8409
GenBank Accession LN512222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K108745-0022-511-G12C-8409
GCCATTTACATTGAATATTTGCTGATTTTCTTAAGGATTTTGAATACGGATCATGATGAA
GTCAAGCTTTTTTCTTTTTCTCTGCTTTCACGTGATAGTTCACATGGACTATAAAGTTTT
GCTGTATATGGAAATACACAGATTTTAGGTAAAAGTTATTTGAAAATCAGGAGATTTTCT
GACGTTTTTCTTTTGTTTGAGAATTTGGATTCAATGAAAGTAGTCAAGAATTCAAATCAA
TGTTTTAAATGCAGCATTTAACATTAAACACCTTTTGCATTTTGATTGTGGACTGTGTAT
GTATAGTTGATGAATTCTTGAACAGCAAATTGTAACTCTTTGAGCAATGGCAGATAGCAG
ACCCAGGCTAGTTACTGGCTCCCCTGAAGGATATCCGGGTCATGTGGACGGTAGATTAAG
GGACGCGAGGTTGAACAATCCTAAGGGACTCACAGTTGATGATAGAGGAAATATCTATGT
TGCAGATACTGTGAATAATGCTATCAGGAAGATCAGTGAAGCAGGTGATTTT
   
Sequence Name 85-K108746-0022-511-G12C-31cp
GenBank Accession LN514559 [GenBank]
Sequencing primer gene specific primer, 31cp: 5'-AAAAATCACCTGCTTCACTGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K108746-0022-511-G12C-31cp
TGCACATAGATATTTCCTCTATCATCAACTGTGAGTCCCTTAGGATTGTTCAACCTCACG
TCCCTTAATCTACCGTCCACATGACCCGGATATCCTTCAGGGGAGCCAGTAACTAGCCTG
GGTCTGCTATCTGCCATTGCTCAAAGAGTTACAATTTGCTGTTCAAGAATTCATCAACTA
TACATACACAGTCCACAATCAAAATGCAAAAGGTGTTTAATGTTAAATGCTGCATTTAAA
ACATTGATTTGAATTCTTGACTACTTTCATTGAATCCAAATTCTCAAACAAAAGAAAAAC
GTCAGAAAATCTCCTGATTTTCAAATAACTTTTACCTAAAATCTGTGTATTTCCATATAC
AGCAAAACTTTATAGTCCATGTGAACTATCACGTGAAAGCAGAGAAAAAGAAAAAAGCTT
GACTTCATCATCATCCGTATTCAAAATCCTTAAGAAAATCAGCAAATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11