Sequences from confirmation amplicon


Confirmation sequences for line 512A09 and hit At1g69630 are shown.
Line ID 512A09
Locus At1g69630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26192864
Primers used for PCR KS16 - 8474, show primer details
   
Sequence Name 35-K026486-022-512-A09C-8409
GenBank Accession LN501685 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K026486-022-512-A09C-8409
CAATTGAATATATCCTGTAATGATGGGTCATACTCGTCATCACAATCATACTTCAAGATA
AATTTTTGTAAGAATGATTCACTGTTGAAATCGAGAAAGCTATCAACAAAACTCAAGAAT
GTGTTGAACTCTTGGAAATCAGTATTGTCTAGGTCTAAACCAGGTACGTGTTTCCAGAGA
TTTCTCCATCTACGGGATAAAACACTAGTCTTAACAACATCTTTTGTGGGAAGATTCAAG
AGTACCTCACATAGCAAACAATCTGGTAATTTGCTTATCCAATCAACTTCATCAGATCCT
TTCGCACGAACATGTTTCTCTCTATCTTCATCCATGAAAAGAATCATCATATCGTATCGA
TAAGAAACCCTCTTAAACTTAAGAGAGTATCGAATCGACAGTTGTACAAGAAAAAAACGA
GTCTACAGTTAATACGACATCGTTTTATAGTATAAATCGCATTGTATTTGTATAACGGGC
CTTATGGGCTTTAGAGAAATAAGAACATAAAAGAAAACGCATTATAATTTGAAACGATGA
GTTTGATGAAGAGATCATGTAAACATGTAGTTGTTGAACAACTTTGAGATCT
   
Sequence Name 36-K026486-022-512-A09C-KS16
GenBank Accession LN502080 [GenBank]
Sequencing primer gene specific primer, KS16: 5'-ACACAACCACATCAGATCTCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K026486-022-512-A09C-KS16
AACTACATGTTTACATGATCTCTTCATCAAACTCATCGTTTCAAATTATAATGCGTTTTC
TTTTATGTTCTTATTTCTCTAAAGCCCATAAGGCCCGTTATACAAATACAATGCGATTTA
TACTATAAAACGATGTCGTATTAACTGTAGACTCGTTTTTTTCTTGTACAACTGTCGATT
CGATACTCTCTTAAGTTTTTGAGGGTTTCTTATCGATTCGATATTTTTTTTCTTTTCATG
GATGAAGATAGAGAGAAACATGTTCGTGCGAAAGGATCTGATGAAGTTGATTGGATAAGC
AAATTACCAGATTGTTTGCTATGTGAGGTACTCTTGAATCTTCCCACAAAAGATGTTGTT
AAGACTAGTGTTTTATCCCGTAGATGGAGAAATCTCTGGAAACACGTACCTGGTTTAGAC
CTAGACAATACTGATTTCCAAGAGTTCAACACATTCTTGAGTTTTGTTGATAGCTTTCTC
GATTTCAACAGTGAATCATTCTTACAAAAATTTATCTTGAAGTATGATTGTGATGACGAG
TATGACCCATCATTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATG

Last Updated on 06.12.2018 16:11