Sequences from confirmation amplicon


Confirmation sequences for line 512G01 and hit At3g03740 are shown.
Line ID 512G01
Locus At3g03740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:937265
Primers used for PCR 16pk - 8474, show primer details
   
Sequence Name 58-K115187-0022-512-G01C-16pk
GenBank Accession LN509787 [GenBank]
Sequencing primer gene specific primer, 16pk: 5'-CCAATTATTACACGGAAAGCGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K115187-0022-512-G01C-16pk
ACATAGTATTCGACAGTGGAAACACTAAGTTACAAGATCTCCGCTTCATGATGTCTGATC
CTAACTTCAGCTTACTCGGATTGTCCTTTGGCTTTTTTATTACCTTTTATTTGCTCAATC
TTCTAGTTCTGCCATTGGGTCATCACTGTTATTGTTGTCATTATTGTTTCTACCGCTTCC
ATTAGCGTTCACAACTTCGACCCAAACACTCTGGCTTCTCTCTGCTCCTCCATTGATATC
TCCCCAAGTCTGTGCTTGTCTTCCGTTTGTCTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 22-K115186-0022-512-G01C-8409
GenBank Accession LN496238 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115186-0022-512-G01C-8409
TACATTGAGACAAACGGAAGACAAGCACAGACTTGGGGAGATATCAATGGAGGAGCAGAG
AGAAGCCAGAGTGTTTGGGTCGAAGTTGTGAACGCTAATGGAAGCGGTAGAAACAATAAT
GACAACAATAACAGTGATGACCCAATGGCAGAACTAGAAGATTGAGCAAATAAAAGGTAA
TAAAAAAGCCAAAGGACAATCCGAGTAAGCTGAAGTTAGGATCAGACATCATGAAGCGGA
GATCTTGTAACTTAGTGTTTCCACTGTCGAATACTATGTGGATCATAGGAGACGATTTAG
TATTGTTGTTGTAATACGCTTTCCGTGTAAATAATTGG

Last Updated on 06.12.2018 16:11