Sequences from confirmation amplicon


Confirmation sequences for line 512H01 and hit At2g17340 are shown.
Line ID 512H01
Locus At2g17340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7542275
Primers used for PCR 57ns - 8474, show primer details
   
Sequence Name 63-K114626-0022-512-H01C-57ns
GenBank Accession LN511045 [GenBank]
Sequencing primer gene specific primer, 57ns: 5'-CATTCCAATCTTGAGAGAATCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K114626-0022-512-H01C-57ns
GACCCTGATAGAACAGCCAAGAAGTCAGTTAACAAAACCAAGACTAGTTAAATTGTTAAC
TCGCGTTTCACTTTCAAACTATAAATGGGACTTACCATGCCTTCTACGATGACTAAGTCG
GCATCAGATGAAAGGTAAGCAAGCTCCTGTGATACTCTTGAGAGATCGATAACCTAAAAC
AGATTTTTGGGAGGGAGAAAAAAAGTAAGAATCCAATGAATAAAAGTATAAAACCATCTA
TATATCTTTCAGGGAGCTTCTAATGGCTTACTGGTAAGTCATTCCCCGAATTTGCAATCA
GAAGCTTCGAAGTATCAACACCTAGCAATTGGCCATTTTCATCCTTCAACTGTAAATTCA
GACTAAAAAATTAGATTCGCTTTTAAAAGCTGTGTCCAATATATACAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCC
   
Sequence Name 63-K114625-0022-512-H01C-8409
GenBank Accession LN511044 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K114625-0022-512-H01C-8409
TTACATTGAATATATCCTGTATATATTGGACACAGCTTTTAAAAGCGAATCTAATTTTTT
AGTCTGAATTTACAGTTGAAGGATGAAAATGGCCAATTGCTAGGTGTTGATACTTCGAAG
CTTCTGATTGCAAATTCGGGGAATGACTTACCAGTAAGCCATTAGAAGCTCCCTGAAAGA
TATATAGATGGTTTTATACTTTTATTCATTGGATTCTTACTTTTTTTCTCCCTCCCAAAA
ATCTGTTTTAGGTTATCGATCTCTCAAGAGTATCACAGGAGCTTGCTTACCTTTCATCTG
ATGCCGACTTAGTCATCGTAGAAGGCATGGTAAGTCCCATTTATAGTTTGAAAGTGAAAC
GCGAGTTAACAATTTAACTAGTCTTGGTTTTGTTAACTGACTTCTTGGCTGTTCTAATCA
GGGTCGTGGAATTGAGACAAACCTTTATGCTCAGTTCAAGTGTGATTCTCTCAAGAATGG
AATG

Last Updated on 06.12.2018 16:11