Sequences from confirmation amplicon


Confirmation sequences for line 513B10 and hit At5g60230 are shown.
Line ID 513B10
Locus At5g60230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24250637
Primers used for PCR NG56 - 8474, show primer details
   
Sequence Name 69-K102370-0022-513-B10C-8409
GenBank Accession LN512167 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K102370-0022-513-B10C-8409
TTACATTGGAGAAGAAGTAGACACATGGCTCTACATGAAATCAAAAAGACCAAACTTTCC
AATGTTTTTCAAAGCTTATTCACATTTACGATCGAAGAACTGGGTTTTGAGATCAGGGTT
GCAATACGGTGTGGATTTTGTGGCATACCGTCACCATCCATCTCTTGTCCACTCTGAATA
CTCGGTTTTAGTTCAGTCTGGTGACAGTGATCGGTTAAGAGTGTGGTCAGATATCCATTG
TGCTGTTAGGTTAAGCGGAAGTGTTGCGAAAACGTTGTTGACTCTCTACGTTAATGGTAA
CTTCAAAGGAGAGGATGTGAATCTACTTGTATGTTTGGAGAACTTCACAGTGGAAGAACA
AACGATAAGTAGATGGAGCCCAGAACTCAGTCGTGAAGATCAATCCACAAATTCAAAACA
ACATGTT
   
Sequence Name 69-K102371-0022-513-B10C-NG56
GenBank Accession LN512168 [GenBank]
Sequencing primer gene specific primer, NG56: 5'-AACATGTTGTTTTGAATTTGTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K102371-0022-513-B10C-NG56
TTATCGTTTGTTCTTCCCTGTGAAGTTCTCCAACATACAAGTAGATTCACATCCTCTCCT
TTGAAGTTACCATTAACGTAGAGAGTCAACAACGTTTTCGCAACACTTCCGCTTAACCTA
ACAGCACAATGGATATCTGACCACACTCTTAACCGATCACTGTCACCAGACTGAACTAAA
ACCGAGTATTCAGAGTGGACAAGAGATGGATGGTGACGGTATGCCACAAAATCCACACCG
TATTGCAACCCTGATCTCAAAACCCAGTTCTTCGATCGTAAATGTGAATAAGCTTTGAAA
AACATTGGAAAGTTTGGTCTTTTTGATTTCATGTAGAGCCATGTGTCTACTTCATTCTCC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATAATGGTCA
ATA

Last Updated on 06.12.2018 16:11