Sequences from confirmation amplicon


Confirmation sequences for line 514C03 and hit At4g25160 are shown.
Line ID 514C03
Locus At4g25160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12903383
Primers used for PCR 36qv - 8474, show primer details
   
Sequence Name 26-K115806-0022-514-C03C-36qv
GenBank Accession LN498070 [GenBank]
Sequencing primer gene specific primer, 36qv: 5'-TTTGGAATGATTCTTCTTCAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115806-0022-514-C03C-36qv
ATTTCGTTGAGAGCGCAATGGATAGTAATGATGAGTTCCTTAAGATCTTAGATCAGAAAG
CCGGTAACTGGCCGATTGAAGAAACCCGGGAATTGGCCGCATTAGCTTTGTGTTGTACAG
AGCTACGCGGGAAAGATAGACCTGATTTGAAAGATCAGATTCTTCCAGCGCTCGAAAACC
TAAAGAAAGTAGCTGAAAAGGCAAGGAACTCATTTTCCGGCGTATCGACACAGCCTCCCA
CTCATTTCATCTGCCCATTACTTAAGGTAAACTCTATATAAAACCTTGTTCAATGTTGAA
ACATCTCATCTCAGTGTGTTGTGGTGAAAATGTGTGAGCTTGTGTGGTATAATGCAGGAC
GTGATGAACGAGCCGTGCGTTGCAGCAGACGGATACACATACGACCGTCATGCGATAGAG
GAGTGGCTTAAGGAGCACAACACATCGCCGATGACGGATTCACCGTTGCATAGCAAGAAC
CTTTTACCCAATTATACTCTTTACACAGCCATCATGGAATGGAGGTCTACATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K115805-0022-514-C03C-8409
GenBank Accession LN488488 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115805-0022-514-C03C-8409
TGATGGCTGTGTAAGAGTATAATTGGGTAAAAGGTTCTTGCTATGCAACGGTGAATCCGT
CATCGGCGATGTGTTGTGCTCCTTAAGCCACTCCTCTATCGCATGACGGTCGTATGTGTA
TCCGTCTGCTGCAACGCACGGCTCGTTCATCACGTCCTGCATTATACCACACAAGCTCAC
ACATTTTCACCACAACACACTGAGATGAGATGTTTCAACATTGAACAAGGTTTTATATAG
AGTTTACCTTAAGTAATGGGCAGATGAAATGAGTGGGAGGCTGTGTCGATACGCCGGAAA
ATGAGTTCCTTGCCTTTTCAGCTACTTTCTTTAGGTTTTCGAGCGCTGGAAGAATCTGAT
CTTTCAAATCAGGTCTATCTTTCCCGCGTAGCTCTGTACAACACAAAGCTAATGCGGCCA
ATTCCCGGGTTTCTTCAATCGGCCAGTTACCGGCTTTCTGATCTAAGATCTTAAGGAACT
CATCATTACTATCCATTGCGCTCTCAACGAAATGTGTCAATGCAATAGCAGGTTTTGCTG
TGAGAAGCTGAAGAAGATAANTTCCA

Last Updated on 06.12.2018 16:11