Sequences from confirmation amplicon


Confirmation sequences for line 514G05 and hit At1g60070 are shown.
Line ID 514G05
Locus At1g60070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22145228
Primers used for PCR 37sn - 8474, show primer details
   
Sequence Name 51-K117387-0022-514-G05C-37sn
GenBank Accession LN507619 [GenBank]
Sequencing primer gene specific primer, 37sn: 5'-TCTCCTGTAAGCAATACAACAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117387-0022-514-G05C-37sn
ATTTTCTCGCGGCATATTATAAGAAAAATCAGGGACGTGAAATGAGTTCATGCGTGATTA
TAACCAGAAAATCTAGAATCAACTAGCTAGGTAGGAGTTGGCATACTCTGAACAGGATGG
GAAACGAGATGAGATCTTTAACAGAGCAATGAGTGCCATTGCCTTAGTTGTGACATCTGA
TAAATGATGCTTAATAGCATTCTCGACAACATCCACTGCATCTGATTCTGTTACCTGATT
ACAGAAGCTTGAGAACACAAATAAATATCTATCCTCCTAATATCTCCTAATATCTATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CACGGGTTATTAT
   
Sequence Name 24-K117386-0022-514-G05C-8409
GenBank Accession LN497162 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117386-0022-514-G05C-8409
TTGAATAGATATTAGGAGATATTAGGAGGATAGATATTTATCTGTGTTCTCAAGCTTCTG
TAATCAGGTAACAGAATCAGATGCAGTGGATGTTGTCGAGAATGCTATTAAGCATCATTT
ATCAGATGTCACAACTAAGGCAATGGCACTCATTGCTCTGTTAAAGATCTCATCTCGTTT
CCCATCCTGTTCAGAGTATGCCAACTCCTACCTAGCTAGTTGATTCTAGATTTTCTGGTT
ATAATCACGCATGAACTCATTTCACGTCCCTGATTTTTTCTTATTAATTATGCCGCGATG
AAAATTAGTCAATGATACTTCTCACCAGTGTTGGGCTTGTTGTATTGCTTACAGGAGA

Last Updated on 06.12.2018 16:11