Sequences from confirmation amplicon


Confirmation sequences for line 514H03 and hit At2g36530 are shown.
Line ID 514H03
Locus At2g36530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15322934
Primers used for PCR 37bk - 8474, show primer details
   
Sequence Name 27-K107548-0022-514-H03C-37bk
GenBank Accession LN498416 [GenBank]
Sequencing primer gene specific primer, 37bk: 5'-GGAGCTTCCACTGGTATCTATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K107548-0022-514-H03C-37bk
CTTGGAAGGGTGTATCTAAGGTGAGAGACTGCGATCTGTTCTGTGTGTTTTTTTAGTGTT
AGTAGAGTTATTTCAATTGATCATGTTGACTGATTCGTTATTTGTTTATATCACTAGGCT
GTTGGCAATGTGAACAACATCATCGGGCCAGCACTTATTGGAAAGGTGAGTGACTCACTT
TAAGCTACTCTCAGAACATCAAATTTGTGTCTAATTCTATGCGCTAGGCTCACTCATTTT
ATTTTTGTTGCTTTTATTATTTCTTGCAGGACCCAACTCAGCAGACTGCTATTGACAACT
TCATGGTCCATGAACTTGACGGAGATGGTGAAGGACAAAAATTCAGGGTATGATGGTCAA
TATGGAGAAAAAGCAATGAGTATATGTGATGGTGATGGTGAAGGACAAAAATTTACACAA
TTTTGTCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTCCTGTAGATAAGTAATG
TATGTCCTTATCTTGAATTAGTTATCACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 11-K107547-0022-514-H03C-8409
GenBank Accession LN491142 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K107547-0022-514-H03C-8409
AGATAGGACTACATTACTTATCTACAGGAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGACAAAATTGTGTAAATTTTTGTCCTTCACCATCACCATCACATATACTCATTGCT
TTTTCTCCATATTGACCATCATACCCTGAATTTTTGTCCTTCACCATCTCCGTCAAGTTC
ATGGACCATGAAGTTGTCAATAGCAGTCTGCTGAGTTGGGTCCTGCAAGAAATAATAAAA
GCAACAAAAATAAAATGAGTGAGCCTAGCGCATAGAATTAGACACAAATTTGATGTTCTG
AGAGTAGCTTAAAGTGAGTCACTCACCTTTCCAATAAGTGCTGGCCCGATGATGTTGTTC
ACATTGCCAACAGCCTAGTGATATAAACAAATAACGAATCAGTCAACATGATCAATTGAA
ATAACTCTACTAACACTAAAAAAACACACAGAACAGATCGCAGTCTCTCACCTTAGATAC
ACCCTTTCCAAGGTAGTCAGATCCTCCATCCCTCAGCTCAAGAGCCTCATAGATACCATG
GNAAAGCTC

Last Updated on 06.12.2018 16:11