Sequences from confirmation amplicon


Confirmation sequences for line 516G02 and hit At4g02880 are shown.
Line ID 516G02
Locus At4g02880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1278717
Primers used for PCR PF15 - 8474, show primer details
   
Sequence Name 54-K102872-0022-516-G02C-8409
GenBank Accession LN508440 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K102872-0022-516-G02C-8409
ATTTACATTGTTCACTATGTTTCCGTTCCTGTGTTATACCTTTCTTTCTAATGAGTAATG
ACATCAAACTTCTTCTGTATTGGAATTATGTCCTTGTGAGTCTCTTTATCATCGTTTCGT
CTTTACATTATATGTGCTACTTTTGTCTAATGAGCCTGAAAAGTGGCTCCAATGGTACGC
ACTGGAAGATTTGTTGGCTTCTGGTAGATATAGCGACAGTGTTGAGCTTGTAATATCATG
TCTCTTATTGCTAAATTAGTTCCTTTCTTAACAGAAACCTTCAAAGTTTTTGTTTTTGTT
TTCATTTACCTAATGTACACATACGCTGGCCATGACTAACAACATGTCCAGGCTTAGAGC
ATATTTTTTTCTAGCTTAAATTGTTAACTTGTCATTCAGTAAAATCCGAGAATTGTGAAG
CTCTAATT
   
Sequence Name 54-K102873-0022-516-G02C-PF15
GenBank Accession LN508441 [GenBank]
Sequencing primer gene specific primer, PF15: 5'-AATTAGAGCTTCACAATTCTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K102873-0022-516-G02C-PF15
GTACATTTAGCTAGAAAAAAATGCTCTAAGCCTGGACATGTTGTTAGTCATGCCCAAAGA
GATGTGTACATTAGGTAAATGAAAACAAAAACAAAAACTTTGAAGGTTTCTGTTAAGAAA
GGAACTAATTTAGCAATAAGAGACATGATATTACAAGCTCAACACTGTCGCTATATCTAC
CAGAAGCCAACAAATCTTCCAGTGCGTACCATTGGAGCCACTTTTCAGGCTCATTAGACA
AAAGTAGCACATATAATGTAAAGACGAAACGATGATAAAGAGACTCACAAGGACATAATT
CCAATACAGAAGAAGTTTGATGTCATTACTCATTAGAAAGAAAGGTATAACACAGGAACG
GAAACATAGTTGAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTCACGTCCAAATGGG
Primers used for PCR 29wq - 8474, show primer details
   
Sequence Name 81-K118656-0022-516-G02C-29wq
GenBank Accession LN514121 [GenBank]
Sequencing primer gene specific primer, 29wq: 5'-TCCAAGCGATTAATCTCAGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K118656-0022-516-G02C-29wq
GACTCTCACCGGAAGACTATCACCCTCGAGGTGGAAAGCTCTGACACCATCGACAACGTT
AAGGCCAAGATCCAGGATAAGGAAGGCATTCCTCCGGATCAGCAGAGGCTTATTTTCGCC
GGAAAACAGCTAGAGGATGGCCGTACGTTGGCTGATTACAATATCCAGAAGGAATCCACC
CTCCACTTGGTTCTCAGGCTCCGTGGTGGTATGCAGATTTTCGTCAAGACCTTAACGGGA
AAGACGATTACTCTTGAGGTGGAGAGTTCTGACACCATCGACAACGTCAAGGCCAAGATC
CAAGACAAGGAGGGTATTCCTCCGGACCAGCAGAGGTTGATCTTCGCTGGAAAGCAGTTG
GAGGATGGCAGAACTCTTGCTGACTACAACATCCAGAAGGAGTCCACACTTCATCTTGTT
CTCAGGCTCCGTGGTGGTATGCAGATCTTTGTCAAGACGTTGACTGGAAAGACTATCACT
TTGGAGGTGGAGAGCTCTGACACTATCGACAATGTCAAAGCCAAGATCCAGGACAAAGAG
GGTATCCCACCGGACCAGCAGAGATTGATCTTCGCCGGAAAACAACTTGAAGATGGTAGA
ACTTTGGCTGACTACAACATTCAGAAGGAGTCTACACTTCACTTGGTGTTGCGTCTCCGT
GGAGGTATGCAGATTTTCGTGAAGACTCTCACTGGAAAGACCATTACTCTTGAAGTTGAG
AGCTCCGACACCATTGACAACGTGAAGGCTAAGATCCAGGACAAGGAAGGTATCCCTCCG
GACCAGCAGCGTCTCATCTTCGCTGGAAAACAGCTTGAGGATGGTCGTACTTTGGCCGAC
TACAACATCCAGAAGGAGTCTACCCTTCACTTGGTGCTAAGGCTCCCTGGTGGTTTCTAA
AAACTTATCTCTGTTATGAATCAGAAGAAAGTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAA
   
Sequence Name 55-K118655-0022-516-G02C-8409
GenBank Accession LN508947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118655-0022-516-G02C-8409
CTTCTTCGATTATACAGAGATAAGTTTTTAGAAACCACCACGGAGCCTTATCACCAAGTG
AAGGGTAGACTCCTTCTGGATGTTGTAGTCGGCCAAAGTACGACCATCCTCAAGCTGTTT
TCCAGCGAAGATGAGACGCTGCTGGTCCGGAGGGATACCTTCCTTGTCCTGGATCTTAGC
CTTCACGTTGTCAATGGTGTCGGAGCTCTCAACTTCAAGAGTAATGGTCTTTCCAGTGAG
AGTCTTCACGAAAATCTGCATACCTCCACGGAGACGCAACACCAAGTGAAGTGTAGACTC
CTTCTGAATGTTGTAGTCAGCCAAAGTTCTACCATCTTCAAGTTGTTTTCCGGCGAAGAT
CAATCTCTGCTGGTCCGGTGGGATACCCTCTTTGTCCTGGATCTTGGCTTTGACATTGTC
GATAGTGTCAGAGCTCTCCACCTCCAAAGTGATAGTCTTTCCAGTCAACGTCTTGACAAA
GATCTGCATACCACCACGGAGCCTGAGAACAAGATGAAGTGTGGACTCCTTCTGGATGTT
GTAGTCAGCAAGAGTTCTGCCATCCTCCAACTGCTTTCCAGCGAAGATCAACCTCTGCTG
GTCCGGAGGAATACCCTCCTTGTCTTGGATCTTGGCCTTGACGTTGTCGATGGTGTCAGA
ACTCTCCACCTCAAGAGTAATCGTCTTTCCCGTTAAGGTCTTGACGAAAATCTGCATACC
ACCACGGAGCCTGAGAACCAAGTGGAGGGTGGATTCCTTCTGGATATTGTAATCAGCCAA
CGTACGGCCATCCTCTAGCTGTTTTCCGGCGAAAATAAGCCTCTGCTGATCCGGAGGAAT
GCCTTCCCTTATCTGGATCTTGGCCTTAACGTTGTCGATGGTGTCAGAGCTTTCCACCTC
GAGGGTGATAGTCTTTCCGGTGAGAGTCTAACAAAGATCTGCATCTGTAATTACAAAAAC
T
Primers used for PCR 11wq - 8474, show primer details
   
Sequence Name 29-K118781-0022-516-G02C-8409
GenBank Accession LN499496 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118781-0022-516-G02C-8409
CTTCTTCTGATTATACAGAGATAAGTTTTTAGAACCACCACGGAGCCTTAGCACCAAGTG
AAGGGTAGACTCCTTCTGGATGTTGTAGTCGGCCAAAGTACGACCATCCTCAAGCTGTTT
TCCAGCGAAGATGAGACGCTGCTGGTCCGGAGGGATACCTTCCTTGTCCTGGATCTTAGC
CTTCACGTTGTCAATGGTGTCGGAGCTCTCAACTTCAAGAGTAATGGTCTTTCCAGTGAG
AGTCTTCACGAAAATCTGCATACCTCCACGGAGACGCAACACCAAGTGAAGTGTAGACTC
CTTCTGAATGTTGTAGTCAGCCAAAGTTCTACCATCTTCAAGTTGTTTTCCGGCGAAGAT
CAATCTCTGCTGGTCCGGTGGGATACCCTCTTTGTCCTGGATCTTGGCTTTGACATTGTC
GATAGTGTCAGAGCTCTCCACCTCCAAAGTGATAGTCTTTCCAGTCAACGTCTGGGACAA
AGA
   
Sequence Name 69-K118782-0022-516-G02C-11wq
GenBank Accession LN512290 [GenBank]
Sequencing primer gene specific primer, 11wq: 5'-TCTTTGTCAAGACGTTGACTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K118782-0022-516-G02C-11wq
CGACAATGTCAAAGCCAAGATCCAGGACAAAGAGGGTATCCCACCGGACCAGCAGAGATT
GATCTTCGCCGGAAAACAACTTGAAGATGGTAGAACTTTGGCTGACTACAACATTCAGAA
GGAGTCTACACTTCACTTGGTGTTGCGTCTCCGTGGAGGTATGCAGATTTTCGTGAAGAC
TCTCACTGGAAAGACCATTACTCTTGAAGTTGAGAGCTCCGACACCATTGACAACGTGAA
GGCTAAGATCCAGGACAAGGAAGGTATCCCTCCGGACCAGCAGCGTCTCATCTTCGCTGG
AAAACAGCTTGAGGATGGTCGTACTTTGGCCGACTACAACATCCAGAAGGAGTCTACCCT
TCACTTGGTGCTAAGGCTCCGTGGTGGTTTCTAAAAACTTATCTCTGTTATGAATCAGAA
GAAGTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGGTTTT
ATTA

Last Updated on 06.12.2018 16:11