Sequences from confirmation amplicon


Confirmation sequences for line 516H06 and hit At2g41150 are shown.
Line ID 516H06
Locus At2g41150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17154211
Primers used for PCR CC89 - 8474, show primer details
   
Sequence Name 05-K106633-0022-516-H06C-8409
GenBank Accession LN488369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106633-0022-516-H06C-8409
TTTGAGATTTCTGATGAAGTTCTTGAAATGGCAATTAGCCTATCTGGTTCAATGATCTGG
AAAGTTGACTAAATGATGCAGCTCTCTTGGTAAAGATGGTCACTGTAATTGCAATTTTCT
TGTTGCAGGACTTTTGTAATGCCATCTCGGATGTGCATCAATCCAATACATAACAAGAAG
GGTATACTTAATCGATCCAACAATGAAACTAGAGAGGAAAGGTGAGAGTTTCTTTAAACT
ACTTCAAAAACTTAGGTTTTGTTTTGTTTCCCACTTTTGAACATATGAGGTCTCTCTCTC
TCTCTCCTTTGTATGTTCTTAGTTGGGAAGTGAGCTCTTGTGCAATGGCATCATTGTATG
ATATTGATCTCATCTCTGAAAAAATACGTGTGATCTTGGATGACTCGGAAATATGGCANA
TAATGCTATNGACGAGTATGAAATTGAAAGAACGTG
   
Sequence Name 21-K106634-0022-516-H06C-CC89
GenBank Accession LN495677 [GenBank]
Sequencing primer gene specific primer, CC89: 5'-CCCACGTTCTTTCAATTTCATACT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K106634-0022-516-H06C-CC89
TTCCGAGTCTCCAGATCACAGGTATTTTCTCAGAGATGAGATCAATATCATACAATGATT
CCATTGCACAAGAGCTCACTTCCCAACTAAGAACATACAAAGGAGAGAGAGAGAGAGACC
TCTTGTGTTCAAAAGTGGGAAACAAAACAAAACCTAAGTTTTTGAAGTAGTTTAAAGAAA
CTCTCACCTTTCCTCTCTAGTTTCATTGTTGGATCGATTAAGTATACCCTTCTTGTTATG
TATTGGATTGATGCACATCCGAGATGGCATTACAAAAGTCCTGCAACAAGAAAATTGCAA
TTACAGTGACCATCTTTACCAAGAGAGCTGCATCATTTATTCAACTAACCATCTCATTGA
ACCAGATAGGCTAATTGCCATTTCAAGAACTTCATCAGAAATCTCAAAAGTTTGACATAT
TGGTGGATTTTGCAAAAGTTACCTGTTCAGAAACATGGCTTCTTCAAGGGCACATCTAAG
GCTAGATTTCCTGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11