Sequences from confirmation amplicon


Confirmation sequences for line 517C11 and hit At2g17320 are shown.
Line ID 517C11
Locus At2g17320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7534950
Primers used for PCR 45xk - 8474, show primer details
   
Sequence Name 77-K119123-0022-517-C11C-45xk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 45xk: 5'-TAAATACAATCCGAAAACTGACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K119123-0022-517-C11C-45xk
TATCAATTCGGAGATGATTCCGNTTTCCTCTGTTACCAANCGCCGATTGAAACCAACTAC
AGAGCTTGCACCATTCCTTATAGATTCCCTTCCGATAATCTCAAGAAAGCCACTCCTACT
GAAATCTCCTGGATCAACGTCTTCGCCAATTCCATTCCTTCTTTCAAGTTCCTCTCTCTC
TACTTTGACCTCATTTAGATACTGCTCATGGGTCGAATCAAGTTTTGATTTTGAAACCAA
AAATTGCTTCTTTTAGGCTCATATTCCAATAGTCTGCTTGATTAATTGCAAATTCTGTCA
AAACTCATGAAACTTTTAGCCCTTAAGCTATTGATAGTGTAGTTGCTAGTTGTTGCTTTG
ACTTTCCTCTGTGTTTACTTAACATCACGAGATTGTTTCTGGAAGCTTATAGGAAACAGA
TGGAATCCTAATTTTAACAATAATTATTCTCCATTGTTCTAAAGCTGTTTCATTTGCAGG
AAACGTGCAGAGAGCGATACCAGTGTTCCAGATGCTCCTGCTAGAGCTAAAATTTTTGAA
CAAAGGTAACATATTTTTTTGCCTTTGCGTATTAATGTGGTAATTCTCAAAGTTTTGGTT
TGTAGGCAAATGTTTTGCTAATGTCACCTTCATAAACTAGACGAGTTTTGTGTCCTGTGG
TTTGCGTTTGATATGTCTTACTTTGAATCTGGAAGATATGCGGGATTTCTTGAAGGCTGG
AAGAAAGATCCAGAGAGTAATGGTGGGCCACCAGATGGTGTTGTAAGTCGAAAACACCTT
ATGATCTTTCTCTTTGATGTAGTGAGTTTTGTTTTCATGGATTATTTTGGCTTGTTTTCA
GATTCTCGGCCGAGTTCGTGAGCATTTACTC
   
Sequence Name 54-K119111-0022-517-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K119111-0022-517-C11C-8409
CATTTACATTGAACCTCAAGTGCCCTTGAAAGAAAATTCGAACCTTGACTTTCTTGAATA
TGTCCCTAAATCCTAACTCTCTGAGTAAATGCTCACGAACTCGGCCGAGAATCTGAAAAC
AAGCCAAAATAATCCATGAAAACAAAACTCACTACATCAAAGAGAAAGATCATAAGGTGT
TTTCGACTTACAACACCATCTGGTGGCCCACCATTACTCTCTGGATCTTTCTTCCAGCCT
TCAAGAAATCCCGCATATCTTCCAGATTCAAAGTAAGACATATCAAACGCAAACCACAGG
ACACAAAACTCGTCTAGTTTATGAAGGTGACATTAGCAAAACATTTGCCTACAAACCAAA
ACTTTGAGAATTACCACATTAATACGCAAAGGCAAAAAAATATGTTACCTTTGTTCAAAA
ATTTTAGCTCTAGCAGGAGCATCTGGAACACTGGTATCGCTCTCTGCACGTTTCCTGCAA
ATGAAACAGCTTTAGAACAATGGAGAATAATTATTGTTAAAATTAGGATTCCATCTGTTT
CCTATAAGCTTCCAGAAACAATCTCGTGATGTTAAGTAAACACAGAGGAAAGTCAAAGCA
ACAACTAGCAACTACACTATCAATAGCTTAAGGGCTAAAAGTTTCATGAGTTTTGACAGA
ATTTGCAATTAATCAAGCAGACTATTGGAATATGAGCCTAAAAGAAGCAATTTTTGGTTT
CAAAATCAAAACTTGATTCGACCCATGAGCAGTATCTAAATGAGGGTCAAGTAGAGAGAG
AGGA

Last Updated on 06.12.2018 16:11