Sequences from confirmation amplicon


Confirmation sequences for line 517F04 and hit At1g54370 are shown.
Line ID 517F04
Locus At1g54370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20294478
Primers used for PCR 28iv - 8474, show primer details
   
Sequence Name 63-K113845-0022-517-F04C-28iv
GenBank Accession LN511034 [GenBank]
Sequencing primer gene specific primer, 28iv: 5'-GATATAAAAATGGGCATCAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K113845-0022-517-F04C-28iv
TAAATAGGTTGACCAAATATTGCACACCCAAATACATTGACAGCCCTGCGTGAGAAAAGC
ATTGCAGAACATATAGGTAAATCCGAATCGAAAACCATGGTGATAATGAAGCGATCAGCC
AGGATAAAAAAGCATTTAGTCACCTAGCCACGCCAATAAACAACTGATGTCAGTCATGAA
ACGCAGAAGCAAAAGGTAAAGTCAAGGATTTACAACACTGACTAACACTGCATACAATCA
CTGACGATCAGTAAATGAGGATACAATAGAGAAAAGGCCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATTCC
   
Sequence Name 36-K113844-0022-517-F04C-8409
GenBank Accession LN502302 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K113844-0022-517-F04C-8409
CCTTTTCTCTATAGTATCCCTCATTTACTGATCGTCAGTGATTGTATGCAGTGTTAGTCA
GTGTTGTAAATCCTTGACTTTACCTTTTGCTTCTGCGTTTCATGACTGACATCAGTTGTT
TATTGGCGTGGCTAGGTGACTAAATGCTTTTTTATCCTGGCTGATCGCTTCATTATCACC
ATGGTTTTCGATTCGGATTTACCTATATGTTCTGCAATGCTTTTCTCACGCAGGGCTGTC
AATGTATTTGGGTGTGCATATTTGGTCAACCTATTTAGACAGGAGAACCAGAAGATACCT
ATGAAGCACCAAAAAGCCCTTTGGTATAGTGGTGAGTTGAGCTAATAGCTTTTGTTCTTG
TCTCCTGATGCCTTTTTTTTATA

Last Updated on 06.12.2018 16:11