Sequences from confirmation amplicon


Confirmation sequences for line 517G12 and hit At3g50820 are shown.
Line ID 517G12
Locus At3g50820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18891988
Primers used for PCR 30iv - 8474, show primer details
   
Sequence Name 84-K113669-0022-517-G12C-30iv
GenBank Accession LN514458 [GenBank]
Sequencing primer gene specific primer, 30iv: 5'-TCAACTTCGCATTATCACTCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K113669-0022-517-G12C-30iv
GCACTTCTCTCCAGCCGCCGCAACTTTTCTTCACCGGCGAAGATTGCCGCTTCTCCTTCT
CGTAATGTCCATCTCCGATCAAACCAAACCGTGGGCAAGTCCTTTGGGTTAGACTCTTCA
CAAGCTAGGCTCACGTGCTCTCTCCACTCTGACCTCAAAGACTTCGCTGGAAAATGCTCC
GACGCCGCCAAGATCGCCGGTTTTGCTCTAGCCACCTCTGCTCTCGTTGTCTCGGTATAA
CTTCATATTCTCATTCATCTCACAAACTTAACAAAATTTATAGGCCTTGTGTTTATTGAA
GAAAACAAACTTAAGAAGAAAATATATGTAGATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 53-K113666-0022-517-G12C-8409
GenBank Accession LN508215 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113666-0022-517-G12C-8409
ATATTTTCTTCTTAAGTTTGTTTTCTTCATAAACACAAGGCCTATAAATTTTGTTAAGTT
TGTGAGATGAATGAGAATATGAAGTTATACCGAGACAACGAGAGCAGAGGTGGCTAGAGC
AAAACCGGCGATCTTGGCGGCGTCGGAGCATTTTCCAGCGAAGTCTTTGAGGTCAGAGTG
GAGAGAGCACGTGAGCCTAGCTTGTGAAGAGTCTAACCCAAAGGACTTGCCCACGGTTTG
GTTTGATCGGAGATGGACATTACGAGAAGGAGAAGCGGCAATCTTCGCCGGTTGAAGAAA
AGTTGCGGCGGCTTGGAGAGAAGTTGCCATGATGACAAGACTCAGATGTCAATATGTTTG
TTTGAGTGATATGGGGAAGTTG

Last Updated on 06.12.2018 16:11