Sequences from confirmation amplicon


Confirmation sequences for line 517H08 and hit At4g16141 are shown.
Line ID 517H08
Locus At4g16141 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9132326
Primers used for PCR 24uv - 8474, show primer details
   
Sequence Name 37-K117403-0022-517-H08C-24uv
GenBank Accession LN502763 [GenBank]
Sequencing primer gene specific primer, 24uv: 5'-TCTTTCACTCAACCATTACGACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117403-0022-517-H08C-24uv
GAGCCATGAGAAGTACTGGCGGCTCGTTCCTCTTCCCCAAGTTTCCTCCATAGCCTCTTC
TTCTCCACAGCCGATCTCTTCATCGCCGGTACCTTAAACCCAAAATCCAAAAATCTACCA
ACCCTTTTCACGTTCTTCTTGTTATTATTGCTGCTACTGGAATTCTCAGGATCTCTCTTG
ATTTTGTTCTTGACATTTCCTGGTTCACCTTTGGCAATCTTGACAATTCCCGGTTCACAT
TTGGCAATCTTAACATTAACTGGTTCGCCTTTTCCGGTCTTGACATTTCGGCTCTCAAGA
CCAAGATTGTTATTACTTTTGCTCTTGATTTTAATATCATCTTGTCTAATTCCAAGAGCT
GCTTGTCTCTTCTTTCTGCTCTTGATACCACACGCATTGCACAATGACTGCAAACAAAGT
CAATCCATGATCAGACCAGAGACATATTCAATTGATCCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 13-K117402-0022-517-H08C-8409
GenBank Accession LN492229 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117402-0022-517-H08C-8409
ATATCCTGGATCAATTGAATATGTCTCTGGTCTGATCATGGATTGACTTTGTTTGCAGTC
ATTGTGCAATGCGTGTGGTATCAAGAGCAGAAAGAAGAGACAAGCAGCTCTTGGAATTAG
ACAAGATGATATTAAAATCAAGAGCAAAAGTAATAACAATCTTGGTCTTGAGAGCCGAAA
TGTCAAGACCGGAAAAGGCGAACCAGTTAATGTTAAGATTGCCAAATGTGAACCGGGAAT
TGTCAAGATTGCCAAAGGTGAACCAGGAAATGTCAAGAACAAAATCAAGAGAGATCCTGA
GAATTCCAGTAGCAGCAATAATAACAAGAAGAACGTGAAAAGGGTTGGTAGATTTTTGGA
TTTTGGGTTTAAGGTACCGGCGATGAAGAGATCGGCTGTGGAGAAGAAGAGGCTATGGAG
GAAACTTGGGGAAGAGGAACGAGCCGCAGTACTTCTCATGGCTCTTTCTTGTGGTTGAGA
TTTTGTCTCTTAACCCTTTTTTTTTGATGTGTCGTAATGGTGAAAGGAAAAAAA

Last Updated on 06.12.2018 16:11