Sequences from confirmation amplicon


Confirmation sequences for line 518A09 and hit At3g49720 are shown.
Line ID 518A09
Locus At3g49720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18440624
Primers used for PCR 30fb - 8474, show primer details
   
Sequence Name 25-K111225-0022-518-A09C-30fb
GenBank Accession LN497501 [GenBank]
Sequencing primer gene specific primer, 30fb: 5'-GTGACTATTCTTGCACAGCAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K111225-0022-518-A09C-30fb
TAGAGGCTTAGGAGAGGCATGCGCAAAGTCTTGCATGTGGGCCCTGACACATGCTCAGTG
GTTTCCAGTCTATTGAAAGAAGAAGAGACTGAAGCATGGGGTGTTGAACCATATGATATT
GAGGATGCAGATTCTCACTGCAAGAGTTTTGTGAGCAAAGGTCTTGTACGTGTTGCTGAT
ATCAAGTTTCCTCTGCCCTACCGGGCAAAATCCTTCTCTCTTGTGATTGTGTCAGATGCT
CTGGATTATCTCTCACCCAAGTACCTGAACAAGACCGTTCCTGAACTCGCAAGGGTGGCT
TCAGACGGTGTGGTTCTTTTTGCAGGTAAACTATCATAAAACTTGAGAAAAAGGGTTGCT
AATAAATGTCTTTCAAGTTTTGGTTTCTAATTCTTGAACTTCAACTGCTCTAGGTCTCCC
TGGTCAGCAGAGAGCTAAAGTTATCCGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 09-K111224-0022-518-A09C-8409
GenBank Accession LN490238 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K111224-0022-518-A09C-8409
TCGGACTGAGCCATTTCATTGAATATATCCGGATAACTTTAGCTCTCTGCTGACCAGGGA
GACCTAGAGCAGTTGAAGTTCAAGAATTAGAAACCAAAACTTGAAAGACATTTATTAGCA
ACCCTTTTTCTCAAGTTTTATGATAGTTTACCTGCAAAAAGAACCACACCGTCTGAAGCC
ACCCTTGCGAGTTCAGGAACGGTCTTGTTCAGGTACTTGGGTGAGAGATAATCCAGAGCA
TCTGACACAATCACAAGAGAGAAGGATTTTGCCCGGTAGGGCAGAGGAAACTTGATATCA
GCAACACGTACAAGACCTTTGCTCACAAAACTCTTGCAGTGAGAATCTGCATCCTCAATA
TCATATGGTTCAACACCCCATGCTTCAGTCTCTTCTTCTTTCAATAGACTGGAAACCACT
GAGCATGTGTCAGGGCCCACATGCAAGACTTTGCGCATGCCATCTCCATAAGCCTTCTTA
AGTACAGGAATGGCTCTNTGGACTTCTGCTGTGCAAGAATAGTCA

Last Updated on 06.12.2018 16:11