Sequences from confirmation amplicon


Confirmation sequences for line 518D01 and hit At4g31000 are shown.
Line ID 518D01
Locus At4g31000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15105617
Primers used for PCR 21wz - 8474, show primer details
   
Sequence Name 42-K119070-0022-518-D01C-21wz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21wz: 5'-TGCAATGTTAAATTCATTCTGTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K119070-0022-518-D01C-21wz
ATAGGAACCTTCACTCCATTCGTATGTATTTGGTGTGGAGATTATCTTAAAGAGGACGAA
TACTGAGACTTTCGGTATTAAGACTCTTTGTCTGACTGATAACCAGTCGTTTAACCTTTT
TCTCCAATGCTTAGATATGGAAGGATATANCTGGTTGATCCTCTTTAAAAAAGTAATTGC
TTCAAGTTTATTTATACAAAAGAAAAGCTCTCGGAATTTTTTTGTAGTACTTTCTTAGTC
GATCTCCACAATTTGAGGCCTCCTCTCTGCAGCTTTCTTGCGTATAAAGATACCCCATCT
CAATGCAGCTTTGAGCTTAAGCCATCCCACTACAGCTTTCCCTGCGCCTCTACCTCCTTC
TCTCATATAACCCTTGTCTATCTGAGCTTCGTACTGATCACTGAAACCGTAAATGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCACAATTATT
   
Sequence Name 10-K119069-0022-518-D01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119069-0022-518-D01C-8409
CATTTCAATTGAATATATCCATTTACGGTTTCAGTGATCAGTACGAAGCTCAGATAGACA
AGGGTTATATGAGAGAAGGAGGTAGAGGCGCAGGGAAAGCTGTAGTGGGATGGCTTAAGC
TCAAAGCTGCATTGAGATGGGGTATCTTTATACGCAAGAAAGCTGCAGAGAGGAGGCCTC
AAATTGTGGAGATCGACTAAGAAAGTACTACAAAAAAATTCCGAGAGCTTTTCTTTTGTA
TAAATAAACTTGAAGCAATTACTTTTTTAAAGAGGATCAACCAGTATATCCTTCCATATC
TAAGCATTGGAGAAAAAGGTTAAACGACTGGTTATCAGTCAGACAAAGAGTCTTAATACC
GAAAGTCTCAGTATTCGTCCTCTTTAAGATAATCTCCACACCAAATACATACGAATGGAG
TGAAGGTTCCTATATATATTGACAAAGAAAAATTAAGGATTTATGAACAGAATGAATTAA
ACATTGC

Last Updated on 06.12.2018 16:11