Sequences from confirmation amplicon


Confirmation sequences for line 518D09 and hit At4g19460 are shown.
Line ID 518D09
Locus At4g19460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10611210
Primers used for PCR 15jr - 8474, show primer details
   
Sequence Name 79-K113379-0022-518-D09C-15jr
GenBank Accession LN513850 [GenBank]
Sequencing primer gene specific primer, 15jr: 5'-TTGTAGAAACCCTTGAGCTCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K113379-0022-518-D09C-15jr
CTTTTTCCCTATTCCTTGTAACGTTGCTCCCATGGCCCTGATCCAGCTACTACGAGGTAA
ACATTTGAATACGTTTGGATTATTTTCGCGAAAGCTTCGAAGAGCAATGGATGTCCTTTG
TCTTTGACTAATCTCCCAGCAGCTCCTAAAACAATCGCTGATGAGTTTTCTGGTAACCCT
AATTTTGACCTAAACAGAGTACGTAGCTTCTTGTCTGATGTGAATCCGTTCTCGTCGACT
CCATTGAGGATCACATGAACCCTTTTCTCAGGGATTTGGTAAACGTCTCTTAGCATTTCT
CCGCAGCTATCGCTGATAGCGATGTGGTGAGCGTAGTTGTGGAAGAATCTGATTTCGTCC
AGTATCTTGGGAAGCACGGCACCGTATAGACTTGCCGCAATGCTACATGGCAATGCAGTA
TCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 41-K113377-0022-518-D09C-8409
GenBank Accession LN504148 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K113377-0022-518-D09C-8409
TCTGCATTGCCATGTAGCATTGCGGCAAGTCTATACGGTGCCGTGCTTCCCAAGATACTG
GACGAAATCAGATTCTTCCACAACTACGCTCACCACATCGCTATCAGCGATAGCTGCGGA
GAAATGCTAAGAGACGTTTACCAAATCCCTGAGAAAAGGGTTCATGTGATCCTCAATGGA
GTCGACGAGAACGGATTCACATCAGACAAGAAGCTACGTACTCTGTTTAGGTCAAAATTA
GGGTTACCAGAAAACTCATCAGCGATTGTTTTAGGAGCTGCTGGGAGATTAGTCAAAGAC
AAAGGACATCCATTGCTCTTCGAAGCTTTCGCGAAAATAATCCAAACGTATTCAAATGTT
TACCTCGTAGTAGCTGGATCAGGGCCATGGGAGCAACGTTACAAGGAATTAGGGGAAAAA
GTTTCCATTTTGGGATCTCTAAACCCAAATGAGCTCAAGGTTTTCCTAC

Last Updated on 06.12.2018 16:11