Sequences from confirmation amplicon


Confirmation sequences for line 518D10 and hit At3g47250 are shown.
Line ID 518D10
Locus At3g47250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17400545
Primers used for PCR NG52 - 8474, show primer details
   
Sequence Name 70-K102371-0022-518-D10C-NG52
GenBank Accession LN512357 [GenBank]
Sequencing primer gene specific primer, NG52: 5'-AAATCAGGTCCCTTTCTTTGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K102371-0022-518-D10C-NG52
GGTCCCTGGTGATCTAAACAGGATGGCGTTTAGCTTCTTCAACCTCTCAATAGATAAACC
AGATACATATTGGGCGAAACACAGAGACCGCGGGGCTAAACATCTTCTTGACCTCAACCG
TAAGACCTTCATACCTAGCATGAGATCTATGGGTGAATCAGAAACAACACCATCCTCGAA
ATTTCAACACTCTAAGGGTAAATCCGGAGAAGTATCATCGTCAGAGTCTACATTTCCGTT
GATACTGTCGGCCAAAAGACTTCGCCTCCAAGGGATAAAGTTCAGGTTAAGGAGTGATGC
TGAATCAATATTAGATATAAAATTAAAGAAGAACAAGCTTCAGATACCACTGTTAAGATT
GGATGGTTTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATAGT

Last Updated on 06.12.2018 16:11