Sequences from confirmation amplicon


Confirmation sequences for line 518H09 and hit At3g08900 are shown.
Line ID 518H09
Locus At3g08900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2709410
Primers used for PCR 47nb - 8474, show primer details
   
Sequence Name 55-K114597-0022-518-H09C-47nb
GenBank Accession LN508885 [GenBank]
Sequencing primer gene specific primer, 47nb: 5'-TTCCTTGTCGTTTCAAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114597-0022-518-H09C-47nb
AGCTAGACATTGTGATACCTACGATTCGAAACCTCGACTTCCCTTGAGATGTGGCGACCT
TTCTTTGAGCAGTATCATTTGATCATTGTTCAAGATGGTGATCCTTCGAAAGTCATCAAC
ATTCCAGTAGGGTTTGACTACGAGCTCTACAACAGGAACGACATCAATCGAATCTTGGGT
CCCAAGGCCTCTTGCATTTCCTTCAAGGACTCTGCTTGTCGTTGCTTCGGTTACATGGTC
TCCAAAAAGAAGTACATCTACACCATTGATGATGATTGCTTTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 15-K114596-0022-518-H09C-8409
GenBank Accession LN493077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114596-0022-518-H09C-8409
ATGTACTTCTTTTTGGAGACCATGTAACCGAAGCAACGACAAGCAGAGTCCTTGAAGGAA
ATGCAAGAGGCCTTGGGACCCAAGATTCGATTGATGTCGTTCCTGTTGTAGAGCTCGTAG
TCAAACCCTACTGGAATGTTGATGACTTTCGAAGGATCACCATCTTGAACAATGATCAAA
TGATACTGCTCAAAGAAAGGTCGCCACATCTCAAGGAAGTCGAGGTTTCGAATCGTAGGT
ATCACAATGTCTAGCTCATCTTTCAACATTGGCGTGGGTTTCACGGAAGAATACAATTGC
GCCATTTGAA

Last Updated on 06.12.2018 16:11