Sequences from confirmation amplicon


Confirmation sequences for line 519C04 and hit At5g38120 are shown.
Line ID 519C04
Locus At5g38120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15216067
Primers used for PCR EK04 - 8474, show primer details
   
Sequence Name 76-K021740-022-519-C04C-EK04
GenBank Accession LN513313 [GenBank]
Sequencing primer gene specific primer, EK04: 5'-GACTTCCAAGATTTTTGCCTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K021740-022-519-C04C-EK04
TATTTTGTTTTGTTTGATTTTTGATTTGTTGCATGGCAAAGGTTTCCAGATAAAGAAGCA
GGACAATTTCCGATGGCTTACGTAGCACGAAAGCCTGAGAGTAATCTTTGTGAGAAAAAG
GTTATCGACTTTATTTCTAAACAGGTGCTTTAATGATCAAAGCCATGCATAACTAAGTTC
AATTATATAAAATTTTGTAATTTTAGGTTATTAATTTTTGTTAATGTAATAAACAGGTGG
CACCATATAAGAAAATAAGAAAAGTCGCATTTATAGACTCAATTGTAAATTTGCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
C
   
Sequence Name 75-K021740-022-519-C04C-8409
GenBank Accession LN513158 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K021740-022-519-C04C-8409
ATTTCATTGCAAATTTACAATTGAGTCTATAAATGCGACTTTTCTTATTTTCTTATATGG
TGCCACCTGGTTTATTACATTAACAAAAATTAATAACCTAAAATTACAAAATTTTATATA
ATTGAACTTAGTTATGCATGGCTTTGATCATTAAAGCACCTGTTTAGAAATAAAGTCGAT
AACCTTTTTCTCACAAAGATTACTCTCAGGCTTTCGTGCTACGTAAGCCATCGGAAATTG
TCCTGCTTCTTTATCTGGAAACCTTTGCCATGCAACAAATCAAAAATTCAAACAAAACAA
AATAAACTTCTTGTCGTTTTTATGTTTAAAAGGCAAAAATCNNTGGGAAG

Last Updated on 06.12.2018 16:11