Sequences from confirmation amplicon


Confirmation sequences for line 520H01 and hit At1g32750 are shown.
Line ID 520H01
Locus At1g32750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11846399
Primers used for PCR HF28 - 8474, show primer details
   
Sequence Name 50-K032523-022-520-H01C-HF28
GenBank Accession LN507070 [GenBank]
Sequencing primer gene specific primer, HF28: 5'-TTGAAAGAGATGGTGCAGAATATG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K032523-022-520-H01C-HF28
TAAATTCTCTTTGCTTCCTATTTCACTTTGTTCAGTTGAAGTAAATGCTCTATAGCCACG
TAGCATGCTTTCATATGTCTACATAACCTCAACCTTTGTTTGTTTTCTCTCCTCAATTCA
ATTACCAACAGGTTGGCCTAGCAAACATCTTGGAACGCATAGTGGACACACTAAGACTAA
AAGAAGAAGTGTCACGTCTCTTCTTGAAACCGGTATCCAAGAAGGAAGCTCCAGATTATC
TTGATATCGTGGAAAACCCGATGGATCTCTCGACAATCCGAGATAAGGTACGGAAGATAG
AGTACAGAAACCGAGAACAGTTTAGACACGATGTCTGGCAGATAAAGTACAATGCTCATC
TTTACAACGATGGGCGTAACCCAGGAATACCACCTTTGGCAGATCAGTTACTGGAGATTT
GTGATTACTTGTTGGACGATTATGAAGATCAGTTAAAAGAAGCAGAGAAAGGTATAGCAA
TAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAACTTTATTTCG
ACGTGTCTACAT
   
Sequence Name 50-K032523-022-520-H01C-8409
GenBank Accession LN507069 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K032523-022-520-H01C-8409
TTACAATTGAATATTATTGCTATACCTTTCTCTGCTTCTTTTAACTGATCTTCATAATCG
TCCAACAAGTAATCACAAATCTCCAGTAACTGATCTGCCAAAGGTGGTATTCCTGGGTTA
CGCCCATCGTTGTAAAGATGAGCATTGTACTTTATCTGCCAGACATCGTGTCTAAACTGT
TCTCGGTTTCTGTACTCTATCTTCCGTACCTTATCTCGGATTGTCGAGAGATCCATCGGG
TTTTCCACGATATCAAGATAATCTGGAGCTTCCTTCTTGGATACCGGTTTCAAGAAGAGA
CGTGACACTTCTTCTTTTAGTCTTAGTGTGTCCACTATGCGTTCCAAGATGTTTGCTAGG
CCAACCTGTTGGTAATTGAATTGAGGAGAGAAAACAAACAAAGGTTGAGGTTATGTAGAC
ATATGAAAGCATGCTACGTGGCTATAGAGCATTTACTTCAACTGAACAAAGTGAAATATG
GAAGCAAAGAGAATTTTACCTCTCCTTTTTTGCGTCGTTTTGGTTGAGGCGCATATTCTG
CACCATCTTCTTTTCA
Primers used for PCR 44wn - 8474, show primer details
   
Sequence Name 46-K119004-0022-520-H01C-44wn
GenBank Accession LN506023 [GenBank]
Sequencing primer gene specific primer, 44wn: 5'-GTACATTAGCGGAGAAGAGGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K119004-0022-520-H01C-44wn
AGAGGTAGAGATAGAGAGAGCCANCGCGCCGTCCAATGAGCTGGTGGCACGTGACTCGCA
GCTGAGAGCGGAGGTGCAAGTATGGCAAGAGAGAGCTAAAGCGCACGAAGACGCCGCCGC
GTCGCTGCAGTCTCAGCTCCAGCAAGCCGTTAACCAATGCGCCGGTGGATGTGTATCGGC
GCAGGATAGTAGAGCGGCGGAGGAAGGGCTATTATGCACCACAATCAGCGGAGTGGATGA
CGCCGAGTCGGTGTACGTGGATCCGGAGAGAGTAAAGCGGCCGAATTGCAAAGCTTGCCG
GGAAAGAGAGGCAACGGTGGTTGTGTTGCCGTGTCGGCATCTGAGCATCTGCCCGGGATG
TGACCGGACAGCTTTAGCTTGCCCGTTGTGTCTCACGTTGCGGAATTCAAGTGTTGAAGC
TATCTTTTGCTAAATGGGCCTAATTCAAGCCCATTAGGTTGTTATCGTAAATAGAACTTA
GTAGGTAACACAAATATAAATTTCATTGGTATATGATATACCGTATATCGAATCCACTAG
CTATTAAGGAAGCCCTCTAAATTTTACAACAAAAGAAGTTTTTTTTTTTTTTTTTTACTT
TCCATAACTGGGGATTTTTTACCAAAATTTGGAATATGAAAATTTTCCTTTTCCCCAAAA
ATATTTAACTAGCCTAAAAACCCCTTTTTGGGGGGGCCAAAAAAAGG
   
Sequence Name 29-K119003-0022-520-H01C-8409
GenBank Accession LN499502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K119003-0022-520-H01C-8409
CATTCCCAATGACTGAGAAATTTGTAAGATAGAAATCTGTAACCCTTTCTTTAGGCCCCC
CAAAAATGGGTTTCTTAGCTTAGTTAACTATTTTGTACGAATAGCAACATTTCCATATCC
CATATCTGTGTAAAGAATCCCAAGTAATGAAAAGTAAAAAAAAAAAAAAAAACCTTTTTT
TGTGGAAAAATTAAAGGGGCTCCCTAAAAACCTGGGGAATCCAATAACGGGTATTTCAAA
ACCCAAGAAATTTTTTTTTGGGGTTCCCCACAAATTTCTTTTTACAAAAACACCCAAGGG
GGTTTAAATTGGCCCCTTTTAAAAAAAAAAACTTTCAACCTTTAATTCCCCCAACGGGAA
AAAAAAGGGGAAAGTAAAAGTTGCCGGGCCACTCCCGGGGAAAATGCTAAAATGCCAACC
GGGAAACAAACCCCCGGTTGCCTTTTTTTCCGGGAAAGTTTTGAAATTCGGCCCTTTTAC
TCTCCCGGAACCCAGTAACCCAAACCGGGGGTCTCCCACCCCGCTAATGGGGGGGAAAAA
AACCCTTTCCCCCCCCGTTTAATTATCCGGGCCAAAAACAATTCCCCGGGCCCTTTGTTA
AACGGTTTGTGGAACTAAAAATTGCACCAACCGGGGGGCTTTTTCGGGCGTTTAACCTCT
CTTTGGCCATATTGGCCCCCCCCTT

Last Updated on 06.12.2018 16:11