Genotyping and primer details


Genotyping and primer details for 520H01 - At1g32750
Line ID 520H01
Locus At1g32750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11846399 of line 520H01 Chr1:11846399
   
Insertion allele amplicon 8474 - 44wn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 44wn: 5'-GTACATTAGCGGAGAAGAGGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11845565
   
Insertion allele amplicon 8474 - HF28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HF28: 5'-TTGAAAGAGATGGTGCAGAATATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11846934
   
wt amplicon (over InsPos) 44wn - HF28
wt amplicon start Chr1:11845565
wt amplicon end Chr1:11846934
wt amplicon size 1370 bp
Locus specific primer HF28: 5'-TTGAAAGAGATGGTGCAGAATATG-3'
2nd locus specific primer 44wn: 5'-GTACATTAGCGGAGAAGAGGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11