Sequences from confirmation amplicon


Confirmation sequences for line 521C04 and hit At1g48150 are shown.
Line ID 521C04
Locus At1g48150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17785751
Primers used for PCR CC93 - 8474, show primer details
   
Sequence Name 06-K106611-0022-521-C04C-8409
GenBank Accession LN488827 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106611-0022-521-C04C-8409
TCGGACTGAGCCTTTACATTGAATATATCCTGTCTGAGTAAGAATCATCGAAGATCGTTT
GATGAGAACGAAGAAGCATCTCATGGATTATCTTGAGAAGAAAGAAAAATCACACTCTGT
TTCTAAATCCGATCAAAACCCTAACAACGACAGTGGGTCTTCTTCTTCGTCTTCTCAAAT
TGCTTCTGATTTTGGTCAAAACCCTAGTACCTTGTCTCCGTCTTCCTTAAAAATTGTTTC
TTTTGATCAGAACTCTTACTCCTCACTTGAGCCTTCGTCACAAGTTACTACTTGTTTTGA
TCAAAACCCTGTTTTCTCTGTTGGCAGCGAGTCATCATCTGATCAATCTCGTTACTTGGT
GAATGAAGATTCTGGATTTGTTGACGGCCTCTTATGTGAAACAGAGGAAGAGAATAACGG
AATGCGTCTTCCCCAAGAAACACAAACACAACCGATGTTCACAGAGGAGGATCAGAGCTT
TTGGGAAAACCTAGATGTCGACGACGTGTTTGGTCTTTTTAAGGACGACAACAATCTTGA
GGTTCCTCTTCA
   
Sequence Name 22-K106612-0022-521-C04C-CC93
GenBank Accession LN496129 [GenBank]
Sequencing primer gene specific primer, CC93: 5'-TTGAAGAGGAACCTCAAGATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106612-0022-521-C04C-CC93
CACGTCGTCGACATCTAGGTTTTCCCAAAAGCTCTGATCCTCCTCTGTGAACATCGGTTG
TGTTTGTGTTTCTTGGGGAAGACGCATTCCGTTATTCTCTTCCTCTGTTTCACATAAGAG
GCCGTCAACAAATCCAGAATCTTCATTCACCAAGTAACGAGATTGATCAGATGATGACTC
GCTGCCAACAGAGAAAACAGGGTTTTGATCAAAACAAGTAGTAACTTGTGACGAAGGCTC
AAGTGAGGAGTAAGAGTTCTGATCAAAAGAAACAATTTTTAAGGAAGACGGAGACAAGGT
ACTAGGGTTTTGACCAAAATCAGAAGCAATTTGAGAAGACGAAGAAGAAGACCCACTGTC
GTTGTTAGGGTTTTGATCGGATTTAGAAACAGAGTGTGATTTTTCTTTCTTCTCAAGATA
ATCCATGAGATGCTTCTTCGTTCTCATCAAACGATCTTCGATGATTCTTAACTCAGACAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCA

Last Updated on 06.12.2018 16:11