Sequences from confirmation amplicon


Confirmation sequences for line 521D01 and hit At5g57580 are shown.
Line ID 521D01
Locus At5g57580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23315600
Primers used for PCR 24lx - 8474, show primer details
   
Sequence Name 85-K114263-0022-521-D01C-24lx
GenBank Accession LN514574 [GenBank]
Sequencing primer gene specific primer, 24lx: 5'-CTTACTGATAGCCAAAAGGTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K114263-0022-521-D01C-24lx
CTGCAGTTATAGTTTAGTTTAAGTTAAGATTCACTATCTTCTATTAAAAGTTTATTAGCT
ATGATTCACCATCTTCTTTTGAATCCACTTAAAGAAGAGAGTTATCAAATTTGATTCTTT
CTTTCACTTCTGTGTTCTAAAGGTATATGTTGAGGGGCTGGTGAAGAAAGCATATGAAAA
CTGGAACCTAGTCATAGAGTATGATGGAAAATCTCTTTTAGACTTGAAGCAGCCCCAGAG
GTTGAGTATTACTCACACTAATCTGGAAAATTACTCGACTGCTGCCATTGACCATCCNAT
GCAGATGGTGGCAGGGCATTCGTCTTCAATGCCGCCNAACCAGTCTCCAGTGCTTTCAGA
TTTTGCTATTGGAGGTTTGCATATTGAAATCAATCTCTTGATAATTTGTGTTATAGTTTA
ATACTAAATCTTATATATTTGTTCGATTCAATCAATCTACAGGGTATGATATCTTGGTAG
ATTTCCCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCANAAATGTAGATATC
   
Sequence Name 42-K114262-0022-521-D01C-8409
GenBank Accession LN504548 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114262-0022-521-D01C-8409
TCCTGTCCGGGAAATCTACCAAGATATCATACCCTGTAGATTGATTGAATCGAACAAATA
TATAAGATTTAGTATTAAACTATAACACAAATTATCAAGAGATTGATTTCAATATGCAAA
CCTCCAATAGCAAAATCTGAAAGCACTGGAGACTGGTTAGGCGGCATTGAAGACGAATGC
CCTGCCACCATCTGCATTGGATGGTCAATGGCAGCAGTCGAGTAATTTTCCAGATTAGTG
TGAGTAATACTCAACCTCTGGGGCTGCTTCAAGTCTAAAAGAGATTTTCCATCATACTCT
ATGACTAGGTTCCAGTTTTCATATGCTTTCTTCACCAGCCCCTCAACATATACCTTTAGA
ACACAGAAGTGAAAGAAAGAATCAAATTTGATAACTCTCTTCTTTAAGTGGATTCAAAAG
AAGATGGTGAATCATAGCTNATAAACTTTTAATAGAAGATAGTGAATCTTAACTTAAACT
AAACTATAACTTGCAGCAAAGCAAAGAATGTTGTTATAAGTTAAGCAACATGGAGCAAAC
CTTTTGGCATTNAGTAA

Last Updated on 06.12.2018 16:11