Sequences from confirmation amplicon


Confirmation sequences for line 521G06 and hit At3g57800 are shown.
Line ID 521G06
Locus At3g57800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21411713
Primers used for PCR JL40 - 8474, show primer details
   
Sequence Name 05-K031934-022-521-G06C-8409
GenBank Accession LN488235 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K031934-022-521-G06C-8409
GAGCCTTTACAATTGAATATATCGTAACAGTGGCCTTTATTTAACAGACGCACTCCACTG
CTAACGTTGCTTTCGTCCTACTCTTCTTCTGGTGTAAAAGCTTTTCTTCCACACACATTT
GAGAAAGAAGACGAAGAAGAAGAGAGAATAACTATTCCTTCTTCGCCAAGTTCTCTGAGC
TATAGAAGAAGGAGGGATTTCTTCAGACCGCCATTGTTGCCCACCTTCTCTCCTTTCTAT
TTTTCGCCGCATTTTACTCGCTTACTGACAAAAGTCTTCTACTCAAGTCTCTCTCTCGCT
CTCTTTGGAGGCTCAATTAGGGTTTTGTTGTCGTGAGATTTGATTACACAAATTGCTGAA
TTTGGTTTCGATTATTGGTGTTATTGTTTTCGAAGATTTCCAGTGAGTTTCCGTTTATGG
ATCTGACTGGAGGATTTGGAGCTAGATCCGGCGGTGTTGGACCGTGCCGGGAACCAATAG
GCCTTGAATCGCTACATCTCGGTGACGAATTTCGGCAACTAGTGACGACTTTACCTCCCG
AGAACCCCGGCGGTTCGTTCACGGCTTTGCTTGAGCTTCCACCTACACAAGCAGTGGAGC
TTCTCCATTTCACTGATTCTTCGTCTTCTCAACAAGCGGCAGTGACAGGGATCGGTGGAG
AGATTCCTCCGCCGCTTCACTCTTTCGGTGGGACATTGGCTTTTCCTTCTAACTCAGTTC
TCATGGAGCGAGCAGCTCGTTTCTCGGTGATTGCCACTGAGCAACAAAACGGAAATATCT
CCGGGGAGACTCCGACGAGCTCTGTACCTTCCA
   
Sequence Name 06-K031934-022-521-G06C-JL40
GenBank Accession LN488690 [GenBank]
Sequencing primer gene specific primer, JL40: 5'-TTAGCACTTGAATTGGAAGGTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K031934-022-521-G06C-JL40
TCCGTTTTGTTGCTCAGTGGCATCACCGAGAAACGAGCTGCTCGCTCCATGAGAACTGAG
TTAGAAGGAAAAGCCAATGTCCCACCGAAAGAGTGAAGCGGCGGAGGAATCTCTCCACCG
ATCCCTGTCACTGCCGCTTGTTGAGAAGACGAAGAATCAGTGAAATGGAGAAGCTCCACT
GCTTGTGTAGGTGGAAGCTCAAGCAAAGCCGTGAACGAACCGCCGGGGTTCTCGGGAGGT
AAAGTCGTCACTAGTTGCCGAAATTCGTCACCGAGATGTAGCGATTCAAGGCCTATTGGT
TCCCGGCACGGTCCAACACCGCCGGATCTAGCTCCAAATCCTCCAGTCAGATCCATAAAC
GGAAACTCACTGGAAATCTTCGAAAACAATAACACCAATAATCGAAACCAAATTCAGCAA
TTTGTGTAATCAAATCTCACGACAACAAAACCCTAATTGAGCCTCCAAAGAGAGCGAGAG
AGAGACTTGAGTAGAAGACTTTTGTCAGTAAGCGAGTAAAATGCGGCGAAAAATAGAAAG
GAGAGAAGGTGGGCAACAATGGCGGTCTGAACAAATCCCTCCTTCTTCTATAGCTCAGAG
AACTTGGCGAAGAAGGAATAGTTATTCTCTCTTCTTCTTCGTCTTCTTTCTCAAATGTGT
GTGGAAGAAAAGCTTTTACACCAGAAGAAGAGTAGGACGAAAGCAACGTTAGCAGTGGAG
TGCGTCTGTTAAATAAAGGCCACTGTTACGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCGTTATTATAAAATGAAG
TACATTTTGATAAACGACAAAT

Last Updated on 06.12.2018 16:11