Sequences from confirmation amplicon


Confirmation sequences for line 522A03 and hit At1g01420 are shown.
Line ID 522A03
Locus At1g01420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:155263
Primers used for PCR MZ35 - 8474, show primer details
   
Sequence Name 03-K024135-022-522-A03C-MZ35
GenBank Accession LN487313 [GenBank]
Sequencing primer gene specific primer, MZ35: 5'-CCACATGTAGCAATCATACCAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K024135-022-522-A03C-MZ35
GTCACCTTCATCCCACTCGTCGAGTTAGCAAAGCGACTCCTTGACAATCACGGTTTCACC
GTCACTTTCATCATCCCCGGCGATTCTCCTCCGTCTAAGGCTCAAAGATCCGTTCTCAAC
TCTCTCCCTTCCTCCATAGCCTCCGTCTTCCTCCCTCCCGCCGATCTTTCCGACGTTCCT
TCGACAGCTCGAATCGAAACTCGGATATCGCTCACCGTGACTCGTTCCAACCCGGCGCTC
CGGGAGCTTTTTGGCTCGTTATCGGCGGAGAAACGTCTCCCGGCGGTTCTCGTCGTCGAT
CTATTTGGTACGGATGCGTTCGACGTGGCTGCTGAGTTCCACGTGTCGCCATACATTTTC
TATGCATCAAATGCCAACGTCCTCACGTTTCTGCTTCACTTGCCGAAGCTAGACGAAACG
GTGTCGTGTGAGTTTAGGGAATTAACCGAACCGGTTATTATTCCCGGTTGTGTCCCCATA
ACCGGTAAGGATTTCGTCGATCCGTGTCAAGACCGAAAAGATGAATCATACAAATGGCTT
CTACACAACGTCAAGAGATTCAAAGAAGCTGAAGGGATTCTAGTGAATTCCTTCGTCGAT
TTAGAGCCAAACACTATAAAGATTGTACAAGAACCGGC
   
Sequence Name 04-K024135-022-522-A03C-8409
GenBank Accession LN487752 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K024135-022-522-A03C-8409
TGATACTCATTGCATCGTGTGAACCCGAGTTAACCAACGGCCCAATCAGGTAAACCGGTG
GTTTATCAGGAGCCGGTTCTTGTACAATCTTTATAGTGTTTGGCTCTAAATCGACGAAGG
AATTCACTAGAATCCCTTCAGCTTCTTTGAATCTCTTGACGTTGTGTAGAAGCCATTTGT
ATGATTCATCTTTTCGGTCTTGACACGGATCGACGAAATCCTTACCGGTTATGGGGACAC
AACCGGGAATAATAACCGGTTCGGTTAATTCCCTAAACTCACACGACACCGTTTCGTCTA
GCTTCGGCAAGTGAAGCAGAAACGTGAGGACGTTGGCATTTGATGCATAGAAAATGTATG
GCGACACGTGGAACTCAGCAGCCACGTCGAACGCATCCGTACCAAATAGATCGACGACGA
GAACCGCCGGGAGACGTTTCTCCGCCGATAACGAGCCAAAAAGCTCCCGGAGCGCCGGGT
TGGAACGAGTCACGGTGAGCGATATCCGAGTTTCGATTCGAGCTGTCGAAGGAACGTCGG
AAAGATCGGCGGGAGGGAGGAAGACGGAGGCTATGGAGGAAGGGAGAGAGTTGAGAACGG
ATCTTTGAGCCTTAGACGGAGGAGAATCGCCGGGGATGATGAAAGTGACGGTGAAACCGT
GATTGTCAAGGAGTCGCTTTGCTAACTCGACGAGTGGGATGAGGTGACCTATACCGGGA

Last Updated on 06.12.2018 16:11