Sequences from confirmation amplicon


Confirmation sequences for line 522D04 and hit At3g54020 are shown.
Line ID 522D04
Locus At3g54020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20007931
Primers used for PCR DM16 - 8474, show primer details
   
Sequence Name 79-K101747-0022-522-D04C-8409
GenBank Accession LN513804 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K101747-0022-522-D04C-8409
AATTTATACACATTAACATCAATTATTGGGGTTTCAGTCGGATTTATCACTGAATACAAA
TTCAAATGCGTACCCGATCTGCAGGATCAACACCGTTCCCATTCAAGAGTTTGTGACTCT
CTTCTTTGGTTCTGTCTTTTGAGATCACAGGGAGCAATGCCGTTGTTCGATCAGGCAATT
CTGTTTTAAAACAAAATCATATAAGAAAATTTGTGGGTTTTATGCAAGTAAACACTCAAG
TAAAAGATCAGACCTGGTAATTTCTTGTCGAGGAAGAAGACAACCAAGTTCACAGTATAC
CACGCAACAACCACATCAACAGTGTAATGTTTACGGGACGCAATAATCAAGAGGCTTTGC
AAGATGGCAATGACCCAACCTAACAGCTTTATGAACCTGTGAGAGAGACATTTACAAGCT
ATTATTTTGTCCGGTTATTGTTTTACGATGTGACTAGAGAAACTTTGTACCTTTTAGAAC
CGTATTTCTGGTAAGTGCGGACAAAGACTAGTGTGAATATCATGTGCGATGAGAAAATCA
GGTCTCCACATCCGTATATCACACCACGAGGAACTGAAAAGATCAGCGTATCANCACATA
CGAACTAGAAGAAACATACGTCCAAAAAT
   
Sequence Name 79-K101753-0022-522-D04C-DM16
GenBank Accession LN513805 [GenBank]
Sequencing primer gene specific primer, DM16: 5'-TTCCCTTAATACAATTTTTGGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K101753-0022-522-D04C-DM16
GCTGATCTTTTCAGTTCCTCGTGGTGTGATATACGGATGTGGAGACCTGATTTTCTCATC
GCACATGATATTCACACTAGTCTTTGTCCGCACTTACCAGAAATACGGTTCTAAAAGGTA
CAAAGTTTCTCTAGTCACATCGTAAAACAATAACCGGACAAAATAATAGCTTGTAAATGT
CTCTCTCACAGGTTCATAAAGCTGTTAGGTTGGGTCATTGCCATCTTGCAAAGCCTCTTG
ATTATTGCGTCCCGTAAACATTACACTGTTGATGTGGTTGTTGCGTGGTATACTGTGAAC
TTGGTTGTCTTCTTCCTCGACAAGAAATTACCAGGTCTGATCTTTTACTTGAGTGTTTAC
TTGCATAAAACCCACAAATTTTCTTATATGATTTTGTTTTAAAACAGAATTGCCTGATCG
AACAACGGCATTGCTCCCTGTGATCTCAAAAGACAGAACCAAAGAAGAGAGTCACAAACT
CTTGAATGGGAACGGTGTTGATCCTGCAGATCGGGTACGCATTTGAATTTGTATTCAGTG
ATAAATCCGACTGAAACCCCAATAATTGATGTTAATGTGTATAAATTTCCTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT

Last Updated on 06.12.2018 16:11