SimpleSearch - Line and FST details


Line specific information

 
Line ID 522D04
Vector Used pAC161
Line Availability available as T3 set from NASC (N450056)
Segregation Analysis 50:50:46
Confirmed for Hit At3g54020 At2g27020
Parent of DUPLO pair none
Parent of pair(s) 96695, 96696

Gene hit At3g54020

 
Sequence (A. th genome BLAST matches underlined) 
>28-K020204-022-522-D04-8409
AAAATCTTATGAAAAGATTTAGCGTGGTTTATGCCTCTTTCTCTCTCTTAATATATCACA
CCTGGTAATTTCTTGTCGAGGATGAATACAACCAAGTTCACAGTATTCCTCGCAACATCC
ACTTCAACAGTGTATTGTTTACGGTACGCAATAATCAAGAGGCTTTGCAAGATGGCAATG
ACCCAACCTAACAGCTTTATGAACCTGTGAGAGAGACATTTACAAGCTATTATTTTGTCC
GGTTATTGTTTTACGATGTGGCTATGGGATCCTCCCTATAGTGAG
GenBank Accession BX535934 [GenBank]
Graphic View Graphic view of gene At3g54020
Predicted Position of Insertion Chr3:20007918 - go to primer design
BLAST e Value 2e-91
Hit Clone Code (BAC ID) F5K20
Hit Gene Code At3g54020 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Inositol phosphorylceramide synthase 1
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details

Gene hit At2g27020

 
Sequence (A. th genome BLAST matches underlined) 
>28-K020049-022-522-D04-8409
ATGGGATCCTCCCTATAGAGAGAGGCTTTCTTTGTTCTCAAGCCAGAGACAAGCCAAATC
TTTGGAGCTCCGTCTGACTCCGGGTTGGATATTAAAACGATTCGGGTTGTTTTGTCGGGT
GGGTCGATTCGGTTATTAACGGGCTTTATTAATATTATGATTTGGCCCATTCTTTGGTAA
TCTTTTAACGGTATCGTGAATATTTTTAAGTGATTTTCCTCTTTTGTATAATTTTGAATA
ATCAACCACATGAGGAAGAAAGAAATATTTCTCTTACTCAACAAAATATATGATCATACA
ACACTTCTTTATGAACTCACTATGGGATCCTCCCTATAGTGAGANANGN
GenBank Accession BX535933 [GenBank]
Graphic View Graphic view of gene At2g27020
Predicted Position of Insertion Chr2:11530932 - go to primer design
BLAST e Value 7e-167
Hit Clone Code (BAC ID) T20P8
Hit Gene Code At2g27020 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation 20S proteasome alpha subunit G1
Insertion Classification TS2TE (5')
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37