Sequences from confirmation amplicon


Confirmation sequences for line 522H08 and hit At3g06990 are shown.
Line ID 522H08
Locus At3g06990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2207572
Primers used for PCR 64qi - 8474, show primer details
   
Sequence Name 43-K115550-0022-522-H08C-64qi
GenBank Accession LN504949 [GenBank]
Sequencing primer gene specific primer, 64qi: 5'-GTAAAGTCAATGAGTATTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115550-0022-522-H08C-64qi
TTTATGTATAGCTCNAGAAATAAGAGTTAGCAAGGATCCCAGACTACCAATGGATCAACC
AAAGCAAAAAGTGAAGATTCCCTGCAATCATCCGTTAGAATACATACAACTGAAGGGTGG
AGGATGGCCCGGATCATATGACTATGATATAAATACTTGCGCAGGATGCGATGAAGATTA
TGGCTTCTGCTACTACTGCGCAAGCTGCAGTTTTCGAGCACATTCGGAATGCATCGAGTG
GCCAGACACCATCGACCACCCGAGTCACTCCAGACATCCTCTCAAGAAAGTCTCCCCCGG
GACAATTGACTATACAGATGGCAAATGCCATTTTTGTAGAGAAGAACTTGTTGATCCCAT
GTATCACTGTTCCTTGTGCAACTTCAGCGTAGATGTGAATTGTTGGAGGCATCCGCCACA
GCGTACTATTTATCAACCAAAGAGCCACGAGCATACATTCACCCTCATGCCGAGAAAGAT
CACATTCACTTGTAACGCANGTGGGATGCTTGGTGATTGCAATCCTTACTTTTGTTTTGA
ATGTGGATTCATGCTGCAAAAAAGACTGCATTGATTTGCCCCGAGTGATCAACATCAACC
GTCATGACCACCGCATTTCTCGCACTTATCATCTCGGCCACGGCGATNGGGATTTATGTG
GACTTTGTCGCANAGAGATAGATTGGAGTTTTGGAGCTTTCACTTGCAAGCGTTGTCCTA
GTTATGCTGTTCATTCAAAGTGTGCGGTAAGGAAAGATGTGTGGGATGGAGAAGAACTCG
AAGATGTACCTGAAGAAGAAGAAGAAATTGAAGATCCATACNAGGTAATAAATGATAAAG
AAATCATTCATTTTAGTCATGAAGAACATAGTCTAAGACTTGGCGGTGATGATAATGTTA
CCGGTTATGAAAAGATGTTGTGCGACGCTGGCATCACT
   
Sequence Name 16-K115549-0022-522-H08C-8409
GenBank Accession LN493562 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115549-0022-522-H08C-8409
ATTTCATTGAATATATCCTGGTGATATATTAGCCAGGGATGATCCATCTTTTTCTTCTCT
TCTGGTGAAAAGATGTGGTATAAAGGATGGGGATGCAGTTCATGGTGAAAAGGCTCAGAA
ATGGATCCACACCGCACATCAAATTTATCAAACGTTGTCTTGTACCTGAAGCCATCGAAT
AATTTTTGACAACTTCTACACTTGTAATGGTTGCCAGCCTTATTTTTTACCCTTAGATCA
AGCTTGTCGATGTGCAAGATACTTCGTTTCCTCCGAGGGAGACTAGCACATTCTTTATGA
AGAAAAAACTCGCATTGCACACATTTGAAAAATGGATCAGAACTGATGGGAGTGATGCAA
GCGTCGCACAACATCTTTTCATAACCGGTAACATTATCATCACCGCCAAGTCTTAGACTA
TGTTCTTCATGACTAAAATGAATGATTTCTTTATCATTTATTACCTTGTATGGATCTTCA
ATTTCTTCTTCTTCTTCAGGTACATCTTCGAGTTCTTCTCCATCCCACACATCTNTCCTT
ACCGCACACTTTGAATGAACAGCATAACTAGGACAACGCTTGCAAGTGAAAGCTCCAAAA
CTCCAATCTATCTCTTTGCGACAAAGTCCACATAAATCCCAATCGCCGTGGCCGAGATGA
TAAGTGCGAGAAATGCGGTGGTCATGACGGTTGATGTTGATCACTCGGGGCAAATCAATG
CAGTCTTTTTTGCAGCATGAATCCACATTCAAAACAAAAGTAAGGATTGCAATCACCAAG
CATCCCACATGCGTTACAAGTGAATGTGATCTTTCTCGGCATGAGGGTGAATGTATGCTC
GTGGCTCTNTGGTTGATAAATAGTACGCTGTGGCGGATGCCTCCNACAATTCACATCTA

Last Updated on 06.12.2018 16:11