Sequences from confirmation amplicon


Confirmation sequences for line 523D03 and hit At3g13560 are shown.
Line ID 523D03
Locus At3g13560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4426235
Primers used for PCR CC98 - 8474, show primer details
   
Sequence Name 01-K106588-0022-523-D03C-8409
GenBank Accession LN486586 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K106588-0022-523-D03C-8409
TCGGACTGAGCCTTTCATTGAATATATCCTGACATGAAGACAAGCGGTCAGGACCAGTCT
CTGAAAGAAACTGGGGGATTTTGTTTCCAAATGGGACCTCGGTTTACCCTCTGAGTTTGA
GTGGTGGCTCTAGCTCCGCAGCTCTAAACGGTTCATCGATGTTCTGCGTGGCTAAAGCAG
ATGCTGATGATGATAAGTTGGTTGACGGTTTGAATTGGGCGTGTGGTCAAGGCCGAGCTA
ACTGCGCTGCCATTCAACCGGGCCAACCTTGCTACCTCCCTAACGATGTTAAGAGCCATG
CTTCTTTTGCTTTCAATGACTATTACCAGAAGATGAAAAGTGCAGGTGGAACTTGTGACT
TTGATGGTACGGCTATCACTACGACTAGAGATCCTAGTAAGTTTTTCTCATTTCCTCTGA
TCTATGTGTTGCAATAATTCTATGTTTTCAAGTTGTTTCCTCAGAGTAAATCTCAAATTC
GATGGCTTGTGTGTTTGCAGGCTATAGAA
   
Sequence Name 17-K106589-0022-523-D03C-CC98
GenBank Accession LN493865 [GenBank]
Sequencing primer gene specific primer, CC98: 5'-GTTCTATAGCCTGCAAACACACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K106589-0022-523-D03C-CC98
GACACTTGAAACATAGAATTATTGCAACACATAGATCAGAGGAAATGAGAAAAACTTACT
AGGATCTCTAGTCGTAGTGATAGCCGTACCATCAAAGTCACAAGTTCCACCTGCACTTTT
CATCTTCTGGTAATAGTCATTGAAAGCAAAAGAAGCATGGCTCTTAACATCGTTAGGGAG
GTAGCAAGGTTGGCCCGGTTGAATGGCAGCGCAGTTAGCTCGGCCTTGACCACACGCCCA
ATTCAAACCGTCAACCAACTTATCATCATCAGCATCTGCTTTAGCCACGCAGAACATCGA
TGAACCGTTTAGAGCTGCGGAGCTAGAGCCACCACTCAAACTCAGAGGGTAAACCGAGGT
CCCATTTGGAAACAAAATCCCCCAGTTTCTTTCAGAGACTGGTCCTGACCGCTTGTCTTC
ATTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11