Sequences from confirmation amplicon


Confirmation sequences for line 524C10 and hit At3g28050 are shown.
Line ID 524C10
Locus At3g28050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10444587
Primers used for PCR 68we - 8474, show primer details
   
Sequence Name 82-K118544-0022-524-C10C-68we
GenBank Accession LN514240 [GenBank]
Sequencing primer gene specific primer, 68we: 5'-GTACCTGCCGATGTAGAGAGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K118544-0022-524-C10C-68we
CACAGCATTGCATAGACAATGGCTTGAACATTGCAACAAATAAAGGCCCCTTGATCCGCA
ATGCCCACGTGTGGATAGTATTGTTTATGCAAGATCCAAACAATCCCTAAACATAGAAAT
AGAATCAACCAAAATAAAACCATTTTGCTTGTCAAAAAACTAATCACACTCATAATCTCT
AGGGAAAAACACATGAGTGTAATTATATTCTCACCGAGCAAACGATCGACACTAAAGCTA
TGAGTATGATGGTCAATATGGCTTCATGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 62-K118543-0022-524-C10C-8409
GenBank Accession LN510857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K118543-0022-524-C10C-8409
AGAATATAATTACACTCATGTTGTTTTCCCTAGAGATTATGAGTGTGATTAGTTTTTTGA
CAAGCAAAATGGTTTTATTTTGGTTGATTCTATTTCTATGTTTAGGGATTGTTTGGATCT
TGCATAAACAATACTATCCACACGTGGGCATTGCGGATCAAGGGGCCTTTATTTGTTGCA
ATGTTCAAGCCATTGTCTATTGCAATTGCTGTTGCAATGGGAGTTATCTTCCTGCGAGAT
TCTCTCTA

Last Updated on 06.12.2018 16:11