Sequences from confirmation amplicon


Confirmation sequences for line 526C02 and hit At2g20780 are shown.
Line ID 526C02
Locus At2g20780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8948729
Primers used for PCR CD74 - 8474, show primer details
   
Sequence Name 55-K105791-0022-526-C02C-CD74
GenBank Accession LN508815 [GenBank]
Sequencing primer gene specific primer, CD74: 5'-ATAGTAGACTGTGGCGTCAATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K105791-0022-526-C02C-CD74
CAATCAACATCAGCATTTTCCGTACAACAGGAGATGGGCTAAGAAGCTCACGCCACACGG
GCCTGTCCTCGCTGCCTTCTGTATGTGCAGCCGCCAGTTGTATCTCTGCAAGCCGCTCTT
CCGCTTCGTCATCTCGCTCATTCGTTTTCATGAGCACCTCTCGTGCACTGTCCACTCGGC
CTTTCATCACCAGCCACCTCGGCGACTCAGGGATCACACACAGCGCAAATCCTATGAACA
CCGAAGGAAGAATCCCAACTGCAAGCATGATCCTCCAGCTGATATGCACGGAAAGCCCCG
AGAAAGCGTAGTTCGAAACATAGCCCAGCAAGATCCCTAGATTTATGAAGATCTCAGGGA
ACGAAGTGAAAAATCCTCTAGCTACGGTGGGGGATATCTCGGCGATGTAGACAGGAGCGA
TCATGACACCAAGCCCAATCCCAATGCCGGCTAAAGTTCTCCCTATCATCAAAACCTCGA
AAGACGGAGCGACAGCCATCACGGCAGCACCAGTCTGGAAGACGAGAGCAGCCAAGGCCA
TGGTCCATTTTCTACCGATAGAGTCAAAGAGTGTCCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 63-K105794-0022-526-C02C-8409
GenBank Accession LN510993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K105794-0022-526-C02C-8409
GAGCCTTTACATTGAATATATCCTGGACACTCTTTGACTCTATCGGTAGAAAATGGACCA
TGGCCTTGGCTGCTCTCGTCTTCCAGACTGGTGCTGCCGTGATGGCTGTCGCTCCGTCTT
TCGAGGTTTTGATGATAGGGAGAACTTTAGCCGGCATTGGGATTGGGCTTGGTGTCATGA
TCGCTCCTGTCTACATCGCCGAGATATCCCCCACCGTAGCTAGAGGATTTTTCACTTCGT
TCCCTGAGATCTTCATAAATCTAGGGATCTTGCTGGGCTATGTTTCGAACTACGCTTTCT
CGGGGCTTTCCGTGCATATCAGCTGGAGGATCATGCTTGCAGTTGGGATTCTTCCTTCGG
TGTTCATAGGATTTGCGCTGTGTGTGATCCCTGAGTCGCCGAGGTGGCTGGTGATGAAAG
GCCGAGTGGACAGTGCACGAGAGGTGCTCATGAAAACGAATGAGCGAGATGACGAAGCGG
AAGAGCGGCTTGCAGAGATACAACTGGCGGCTGCACATACAGAAGGCAGCGAGGACAGGC
CCGTGTGGCGTGAGCTTCTTAGCCCATCTCCTGTTGTACGGAAAATGCTGATTGTTGGAT
TTGGAATCCAGTGTTTCCAGCAGATCACAGGAATTGACGCCACAGTCTACTAT

Last Updated on 06.12.2018 16:11