Sequences from confirmation amplicon


Confirmation sequences for line 526H09 and hit At5g39740 are shown.
Line ID 526H09
Locus At5g39740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15904450
Primers used for PCR 24kn - 8474, show primer details
   
Sequence Name 85-K113669-0022-526-H09C-24kn
GenBank Accession LN514572 [GenBank]
Sequencing primer gene specific primer, 24kn: 5'-GACATTGTTAAAGCTTCGGCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K113669-0022-526-H09C-24kn
TCTTACCAACTATGCTGCAGGTATTGAATCAGTTCTATACTTTTGTTTTCCCAATATTTG
ATTCTGTGTGTATGGTTTCTTAAAGACTTAATGTTTTTGTCATGCTAGCTTACTGTACTG
GCCTTCTTTTGGCTCGTCGTGTTCTGAAAATGCTGGAAATGGATGATGAGTACGAGGGAA
ACGTAGAGGTATGTTGTGATCTTTTATTTCATCTTGAAAATAAAGAAACTTGAGAGTTTT
CATCTTTTGATTAATAACTGAATCAAAATTGATGAATCAGGCCACTGAAGAGCACGGTTT
TCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 54-K113666-0022-526-H09C-8409
GenBank Accession LN508542 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113666-0022-526-H09C-8409
TTGAATATATCCTGAAAACCGTGCTCTTCAGTGGCCTGATTCATCAATTTTGATTCAGTT
ATTAATCAAAAGATGAAAACTCTCAAGTTTCTTTATTTTCAAGATGAAATAAAAGATCAC
AACATACCTCTACGTTTCCCTCGTACTCATCATCCATTTCCAGCATTTTCAGAACACGAC
GAGCCAAAAGAAGGCCAGTACAGTAAGCTAGCATGACAAAAACATTAAGTCTTTAAGAAA
CCATACACACAGAATCAAATATTGGGAAAACAAAAGTATAGAACTGATTCAATACCTGCA
GCATAGTTGGTAAGACCAACAGTGAGTCCATACTGAGGAAGCTCATGTGCGTAAGCCGAA
GCTTAAACAATGT

Last Updated on 06.12.2018 16:11